将Minitek系统用于淋病奈瑟菌快速鉴定的适应性研究
Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae.
作者信息
Morse S A, Bartenstein L
出版信息
J Clin Microbiol. 1976 Jan;3(1):8-13. doi: 10.1128/jcm.3.1.8-13.1976.
Abstract
A suspending medium was developed for use with the Minitek system for the confirmatory identification of Neisseria gonorrhoeae, N. meningitidis, and N. lactamica based upon the production of acid from various carbohydrates. The addition of sodium bicarbonate to the medium made negative reactions easier to read. More isolates of N. gonorrhoeae were identified with the suspending medium in the Minitek system than with cystine-Trypticase agar media. With a suitable inoculum size, a positive identification could be made in less than 1 h; most isolates (90,8%) could be identified within 4 h of inoculation. The Minitek system is reliable and easy to use.
摘要
研发了一种用于Minitek系统的悬浮培养基，用于基于多种碳水化合物产酸情况对淋病奈瑟菌、脑膜炎奈瑟菌和乳糖奈瑟菌进行确证鉴定。向培养基中添加碳酸氢钠使阴性反应更易于判读。与胱氨酸胰蛋白酶琼脂培养基相比，Minitek系统中的悬浮培养基鉴定出的淋病奈瑟菌分离株更多。接种量合适时，不到1小时即可做出阳性鉴定；大多数分离株（90.8%）在接种后4小时内即可鉴定出来。Minitek系统可靠且易于使用。
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