WSJM，一种用于淋病奈瑟菌生长的简单化学成分明确的培养基。
WSJM, a simple chemically defined medium for growth of Neisseria gonorrhoeae.
作者信息
Wong T P, Shockley R K, Johnston K H
出版信息
J Clin Microbiol. 1980 Apr;11(4):363-9. doi: 10.1128/jcm.11.4.363-369.1980.
Abstract
The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 10 h in the absence of CO2. Scale-up experiments with the complete medium yielded 5 to 10 g, wet weight, of cells per liter. The complete medium was stable upon storage at 5 degrees C and after lyophilization.
摘要
使用一种名为Wong-Shockley-Johnston培养基（WSJM）的简单化学限定培养基，检测了135株淋病奈瑟菌临床分离株和6株美国典型培养物保藏中心参考菌株的生长需求。这种简单的液体培养基能支持接种量为2×10⁶菌落形成单位/毫升的淋球菌生长，且在无二氧化碳条件下10小时内可产生10¹⁰菌落形成单位/毫升。使用完全培养基进行的放大实验每升可收获5至10克湿重的细胞。完全培养基在5℃储存和冻干后均保持稳定。
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