Stroop D M, Triplett R C, Perrotta G, Roberts L P, Schramm R C, Comer K, Miller-Canfield P, Schumacher H R
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, OH 45267.
Ann Clin Lab Sci. 1994 May-Jun;24(3):250-8.
A side by side comparison of the Cell Dyn 3000 SL and Coulter STKS using identical samples was performed over a four month period in the Clinical Hematology Laboratory at the University of Cincinnati Medical Center. A total of 444 samples comprised of 114 sophomore medical students and 330 randomly selected clinical patients were compared in 20 hemogram parameters, and the ability of each instrument to generate differential suspect flags was analyzed. Correlation coefficients for leukocytes, erythrocytes, hemoglobin, hematocrit, and platelets were 0.99. Correlation coefficients for the Cell Dyn and STKS compared with a 600 cell manual differential were 0.98 and 0.89 for neutrophils, 0.96 and 0.87 for lymphocytes, and 0.72 and 0.48 for monocytes, respectively. Both instruments demonstrated high precision and accuracy by internal and external quality control standards. Each analyzer exhibited strength as a screening instrument for abnormal cell populations. The Coulter STKS had overall sensitivity of 75%, specificity of 94%, 25% false negatives, and 6% false positives. Sensitivity, specificity, false negative and positive rates for the Cell Dyn 3000 SL were 68%, 92%, 32%, and 8%, respectively. Based upon this extensive side by side comparison using an identical sample population, it has been concluded that although statistically significant systematic bias (p < 0.05) exists between the two instruments, both analyzers can adequately support the needs of the clinical hematology laboratory.
在辛辛那提大学医学中心临床血液学实验室，对Cell Dyn 3000 SL和库尔特STKS使用相同样本进行了为期四个月的并排比较。总共444个样本，包括114名大二医学生和330名随机选择的临床患者，对20项血常规参数进行了比较，并分析了每种仪器产生异常怀疑标记的能力。白细胞、红细胞、血红蛋白、血细胞比容和血小板的相关系数为0.99。与600细胞手工分类相比，Cell Dyn和STKS的中性粒细胞相关系数分别为0.98和0.89，淋巴细胞相关系数分别为0.96和0.87，单核细胞相关系数分别为0.72和0.48。根据内部和外部质量控制标准，两种仪器均显示出高精度和准确性。每种分析仪作为异常细胞群体的筛查仪器都有其优势。库尔特STKS的总体灵敏度为75%，特异性为94%，假阴性率为25%，假阳性率为6%。Cell Dyn 3000 SL的灵敏度、特异性、假阴性和阳性率分别为68%、92%、32%和8%。基于使用相同样本群体进行的广泛并排比较，得出的结论是，尽管两种仪器之间存在统计学上显著的系统偏差（p < 0.05），但两种分析仪都能充分满足临床血液学实验室的需求。
