Smith W I, Ladoulis C T, Misra D N, Gill T J, Bazin H
Biochim Biophys Acta. 1975 Apr 8;382(4):506-25. doi: 10.1016/0005-2736(75)90218-7.
Isolated plasma membranes of thymic and splenic lymphocytes from unimmunized and immunized rats of the inbred ACI and F344 strains were analyzed for chemical and enzymatic composition, for membrane protein patterns by polyacrylamide gel electrophoresis and for membrane-associated immunoglobulins. After immunization, the thymic and splenic lymphocyte membranes from F344 rat contained less carbohydrate and higher phospholipid contents than control animals. In both ACI and F344 inbred rat strains the membrane phospholipid to cholesterol weight ratio increased significantly after immunization. The electrophoretic patterns of solubilized membrane proteins and of iodinated external membrane proteins were similar in unimmunized and immunized animals. When thymic and splenic lymphocytes of normal or immunized animals were surface radioidinated, solubilized in Triton X-100, NP-40 or 10 M urea in 1.5 M acetic acid and analyzed by immunoprecipitation, labeled IgM immunoglobulin was recovered from thymic lymphocytes but both labeled IgG and IgM were recovered from splenic lymphocytes. However, when unlabeled isolated plasma membranes were solubilized in 1 percent Triton X-100 and analyzed by immunodiffusion in agarose gels both IgG and IgM were identified in thymic and splenic cells.
对近交系ACI和F344品系未免疫及免疫大鼠的胸腺和脾淋巴细胞的分离质膜进行了化学和酶组成分析、通过聚丙烯酰胺凝胶电泳分析膜蛋白模式以及分析膜相关免疫球蛋白。免疫后，F344大鼠的胸腺和脾淋巴细胞膜中的碳水化合物含量低于对照动物，而磷脂含量更高。在ACI和F344近交大鼠品系中，免疫后膜磷脂与胆固醇的重量比均显著增加。未免疫和免疫动物中，溶解的膜蛋白和碘化外膜蛋白的电泳图谱相似。当正常或免疫动物的胸腺和脾淋巴细胞进行表面放射性碘化、在Triton X-100、NP-40或1.5 M乙酸中的10 M尿素中溶解并通过免疫沉淀分析时，从胸腺淋巴细胞中回收了标记的IgM免疫球蛋白，但从脾淋巴细胞中回收了标记的IgG和IgM。然而，当未标记的分离质膜在1% Triton X-100中溶解并通过琼脂糖凝胶免疫扩散分析时，在胸腺和脾细胞中均鉴定出了IgG和IgM。
