Givskov M, Eberl L, Molin S
Department of Microbiology, Technical University of Denmark, Lyngby.
J Bacteriol. 1994 Aug;176(16):4816-24. doi: 10.1128/jb.176.16.4816-4824.1994.
The responses of Pseudomonas putida KT2442 to various forms of nutrient starvation and stress conditions were examined by two-dimensional polyacrylamide electrophoresis. Carbon deprivation resulted in a temporal expression of two classes of starvation-induced proteins: one class was transiently expressed during the initial phase of starvation, and the second class was expressed throughout the entire starvation period. Proteins of the second class could be further subdivided into proteins induced specifically under conditions of carbon starvation, proteins also induced by conditions of stress created by elevated temperature and osmolarity, and finally proteins that were also induced by conditions of nitrogen as well as phosphate starvation. Addition of glucose to a carbon-starved culture led to initiation of a recovery phase. During this phase, repression of starvation-induced proteins as well as induction of a new class of transiently expressed proteins, referred to as maturation proteins, took place.
通过二维聚丙烯酰胺凝胶电泳研究了恶臭假单胞菌KT2442对各种形式的营养饥饿和胁迫条件的反应。碳源剥夺导致两类饥饿诱导蛋白的时序表达：一类在饥饿初期短暂表达，另一类在整个饥饿期都有表达。第二类蛋白可进一步细分为在碳饥饿条件下特异性诱导的蛋白、也由高温和渗透压升高产生的胁迫条件诱导的蛋白，以及最后由氮和磷饥饿条件诱导的蛋白。向碳饥饿培养物中添加葡萄糖导致恢复阶段的开始。在此阶段，饥饿诱导蛋白受到抑制，同时诱导出一类新的短暂表达蛋白，称为成熟蛋白。
