Yap K L, Lam S K
Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur.
J Virol Methods. 1994 Apr;47(1-2):217-26. doi: 10.1016/0166-0934(94)90079-5.
A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) was developed for the fast-growing and cytopathic cell culture-adapted hepatitis A virus (HAV) strain HM175A.2. Infectivity titration by EIA correlated well with titration by cytopathic effects. The reliability of this assay was demonstrated by close agreement in virus infectivity titers among different assays of the same virus aliquot and between assays of different virus aliquots. HAV infected cell cultures after fixation could be stored for up to 1 week before testing without decline in virus titer.
针对快速生长且适应细胞培养的甲型肝炎病毒（HAV）株HM175A.2，开发了一种基于原位酶免疫测定（EIA）的简单、快速且客观的感染性测定方法。通过EIA进行的感染性滴定与通过细胞病变效应进行的滴定相关性良好。同一病毒等分试样的不同测定之间以及不同病毒等分试样的测定之间，病毒感染性滴度的密切一致性证明了该测定方法的可靠性。固定后的HAV感染细胞培养物在测试前可保存长达1周，病毒滴度不会下降。
