Andzhaparidze A G, Kazachkov Iu A, Balaian M S, Kusov Iu Iu, Poleshchuk V F
Vopr Virusol. 1987 Jul-Aug;32(4):440-8.
The results are presented dealing with experimental inoculation of M. fascicularis and M. arctoides with a strain of hepatitis A virus (HAV), YaM-55, isolated from a M. fascicularis with spontaneous hepatitis A, and parallel experiments on inoculation of these monkey species with HAV preparations (strain HAS-15) obtained as a result of the strain propagation in FRhK-4 cell culture and with specimens from human hepatitis A patients containing HAV particles. The YaM-55 strain of HAV was found to be capable of producing an infectious process quite similar to HA in the inoculated seronegative M. fascicularis and M. arctoides. Two different isolates of HAV derived from humans and the HAS-15 strain of HAV propagated for a long time in FRhK-4 cell culture failed to induce a disease in these monkey species. The classification of the YaM-55 strain with HAV was verified by specific serological studies and by molecular hybridization with cloned cDNA of HAV.
本文呈现了用从一只患自发性甲型肝炎的食蟹猴分离出的一株甲型肝炎病毒（HAV）YaM - 55对食蟹猴和熊猴进行实验接种的结果，以及用在FRhK - 4细胞培养中传代获得的HAV制剂（HAS - 15株）和含有HAV颗粒的人类甲型肝炎患者标本对这些猴种进行接种的平行实验结果。发现HAV YaM - 55株能够在接种的血清阴性食蟹猴和熊猴中引发与甲型肝炎非常相似的感染过程。源自人类的两种不同HAV分离株以及在FRhK - 4细胞培养中长时间传代的HAV HAS - 15株未能在这些猴种中诱发疾病。通过特异性血清学研究以及与HAV克隆cDNA的分子杂交，验证了YaM - 55株属于HAV。
