Dar V S, Ghosh S, Broor S
Hybridoma Laboratory, National Institute of Immunology, New Delhi, India.
J Virol Methods. 1994 Apr;47(1-2):51-8. doi: 10.1016/0166-0934(94)90065-5.
A rapid single-step, sensitive and specific immunogold assay is described for detection of rotavirus. Murine monoclonal antibody (MCA) to the group antigen of rotavirus was dotted on a nitrocellulose paper strip. This was used to capture the rotavirus antigen which was revealed using a conjugate consisting of the MCA conjugated to gold particles. This assay detects 5 x 10(7) particles of rotavirus. It also acutely detected rotavirus from fecal samples, but failed to show any reaction with microscopically confirmed fecal samples containing G. lamblia and E. histolytica. The time taken for the test was approximately 10 min. Its versatility, simplicity, sensitivity and specificity makes it useful for third world countries.
描述了一种用于检测轮状病毒的快速单步、灵敏且特异的免疫金测定法。针对轮状病毒群抗原的鼠单克隆抗体（MCA）点样于硝酸纤维素纸条上。该纸条用于捕获轮状病毒抗原，使用与金颗粒偶联的MCA组成的结合物来显示抗原。该测定法可检测5×10⁷个轮状病毒颗粒。它还能灵敏地从粪便样本中检测到轮状病毒，但对经显微镜确认含有蓝氏贾第鞭毛虫和溶组织内阿米巴的粪便样本未显示任何反应。检测所需时间约为10分钟。其多功能性、简便性、灵敏性和特异性使其对第三世界国家很有用。
