使用以单克隆抗体和胶体金作为标记物的试纸条系统快速检测粪便中的轮状病毒。

Rapid detection of rotavirus in faeces using a dipstick system with monoclonal antibodies and colloidal gold as marker.

作者信息

Fernández D, Valle I, Llamos R, Guerra M, Sorell L, Gavilondo J

机构信息

Division of Immunotechnology and Diagnostics, Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.

出版信息

J Virol Methods. 1994 Jul;48(2-3):315-23. doi: 10.1016/0166-0934(94)90130-9.

DOI:10.1016/0166-0934(94)90130-9

PMID:7989446

Abstract

Rotavirus (RV) is known to be the most common cause of severe diarrhoea in infants and young children, each year leading to an estimated 800,000-900,000 deaths. RV also infects bovines and other species, with high morbidity and mortality. A rapid and simple 'naked-eye' dipstick system was developed to detect human RV in faeces, using nitrocellulose as solid phase, two monoclonal antibodies, and colloidal gold as marker. The system detects 10(4) viral particles (1-2 ng)/g of faeces. For human RV the specificity and sensitivity were 100% when compared with a commercial latex system, and 99% and 98%, respectively, when correlated with traditional RNA-PAGE, and 100% and 98% compared to an ELISA system.

摘要

轮状病毒（RV）是婴幼儿严重腹泻的最常见病因，每年估计导致80万至90万人死亡。RV也感染牛和其他物种，发病率和死亡率很高。利用硝酸纤维素作为固相、两种单克隆抗体以及胶体金作为标记物，开发了一种快速简便的“肉眼”试纸条系统，用于检测粪便中的人RV。该系统可检测出每克粪便中10⁴个病毒颗粒（1 - 2纳克）。对于人RV，与商业乳胶系统相比，其特异性和灵敏度均为100%；与传统RNA - PAGE相关时，特异性和灵敏度分别为99%和98%；与ELISA系统相比，特异性和灵敏度分别为100%和98%。