Fernández D, Valle I, Llamos R, Guerra M, Sorell L, Gavilondo J
Division of Immunotechnology and Diagnostics, Center for Genetic Engineering & Biotechnology (CIGB), Havana, Cuba.
J Virol Methods. 1994 Jul;48(2-3):315-23. doi: 10.1016/0166-0934(94)90130-9.
Rotavirus (RV) is known to be the most common cause of severe diarrhoea in infants and young children, each year leading to an estimated 800,000-900,000 deaths. RV also infects bovines and other species, with high morbidity and mortality. A rapid and simple 'naked-eye' dipstick system was developed to detect human RV in faeces, using nitrocellulose as solid phase, two monoclonal antibodies, and colloidal gold as marker. The system detects 10(4) viral particles (1-2 ng)/g of faeces. For human RV the specificity and sensitivity were 100% when compared with a commercial latex system, and 99% and 98%, respectively, when correlated with traditional RNA-PAGE, and 100% and 98% compared to an ELISA system.
轮状病毒（RV）是婴幼儿严重腹泻的最常见病因，每年估计导致80万至90万人死亡。RV也感染牛和其他物种，发病率和死亡率很高。利用硝酸纤维素作为固相、两种单克隆抗体以及胶体金作为标记物，开发了一种快速简便的“肉眼”试纸条系统，用于检测粪便中的人RV。该系统可检测出每克粪便中10⁴个病毒颗粒（1 - 2纳克）。对于人RV，与商业乳胶系统相比，其特异性和灵敏度均为100%；与传统RNA - PAGE相关时，特异性和灵敏度分别为99%和98%；与ELISA系统相比，特异性和灵敏度分别为100%和98%。
