Vannuffel P, Gilot P, Limbourg B, Naerhuyzen B, Dieterich C, Coene M, Machtelinckx L, Cocito C
Microbiology & Genetics Unit, Medical School, University of Louvain, Brussels, Belgium.
J Clin Microbiol. 1994 May;32(5):1211-6. doi: 10.1128/jcm.32.5.1211-1216.1994.
The previously described (M. De Kesel, P. Gilot, M.-C. Misonne, M. Coene, and C. Cocito, J. Clin. Microbiol., 31:947-954, 1993) a362 recombinant polypeptide of Mycobacterium paratuberculosis was used as reagent for an enzyme-linked immunosorbent assay (ELISA). This ELISA, which is endowed with species specificity with respect to the other mycobacteria, was applied to the analysis of bovine paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle caused by M. paratuberculosis. The distribution of anti-a362 antibodies in the cattle population was analyzed by a computer program (mixture population model) to determine a cutoff value for the test. The prevalence of a362 seropositivity in the Belgian bovine population was estimated to be 12%. The sensitivity of the a362 assay was 70%, as determined with reference sera from the U.S. National Repository of Paratuberculosis Specimens. Some 40% of the animals in the herds with paratuberculosis analyzed were found to be positive by the a362 assay. The latter proved to be 95% specific with respect to both healthy and tuberculous cattle.
将先前描述的（M. 德凯塞尔、P. 吉洛、M.-C. 米松内、M. 科内和C. 科西托，《临床微生物学杂志》，31:947 - 954，1993年）副结核分枝杆菌a362重组多肽用作酶联免疫吸附测定（ELISA）的试剂。这种ELISA对其他分枝杆菌具有种属特异性，用于分析牛副结核病（约内氏病），这是一种由副结核分枝杆菌引起的牛地方性分枝杆菌病。通过计算机程序（混合群体模型）分析牛群中抗a362抗体的分布，以确定该检测的临界值。据估计，比利时牛群中a362血清阳性率为12%。用美国副结核标本国家储存库的参考血清测定，a362检测的灵敏度为70%。在分析的患有副结核病的牛群中，约40%的动物通过a362检测呈阳性。结果证明，该检测对健康牛和患结核病的牛的特异性均为95%。
