Sherman D M, Markham R J, Bates F
J Am Vet Med Assoc. 1984 Jul 15;185(2):179-82.
During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were AGID test-positive for paratuberculosis. Twenty-eight cattle (87.5%) yielded Mycobacterium paratuberculosis from bacterial culture of feces. Four cattle were confirmed as having paratuberculosis on necropsy. One had a negative fecal culture but was lost to follow-up. Thus, 32 of the 33 AGID test-positive cattle (96.9%) were confirmed as paratuberculous by culture or necropsy. Of the 18 cattle that were AGID test-negative, 17 (94%) also were culture-negative after 12 to 20 weeks' incubation of the culture tubes. Direct fecal smears from 29 confirmed paratuberculous cattle were examined. Twenty-three (79.3%) had smears positive for M paratuberculosis. Fifteen culture-negative cattle were examined by direct smear; the results were negative for all.
在19个月的时间里，琼脂凝胶免疫扩散（AGID）试验被用于辅助诊断51头有慢性体重减轻和/或慢性腹泻病史的牛的副结核病。33头牛的AGID试验呈副结核病阳性。28头牛（87.5%）粪便细菌培养分离出副结核分枝杆菌。4头牛经尸检确诊患有副结核病。1头牛粪便培养阴性，但失访。因此，33头AGID试验阳性的牛中有32头（96.9%）经培养或尸检确诊为副结核。18头AGID试验阴性的牛中，17头（94%）在培养管培养12至20周后培养也为阴性。对29头确诊为副结核的牛进行了直接粪便涂片检查。23头（79.3%）涂片副结核分枝杆菌阳性。对15头培养阴性的牛进行了直接涂片检查；结果均为阴性。
