Doi M, Mizuguchi I, Homma M, Tanaka K
Laboratory of Medical Mycology, Nagoya University School of Medicine, Aichi, Japan.
Microbiol Immunol. 1994;38(1):19-23. doi: 10.1111/j.1348-0421.1994.tb01739.x.
Candida strains were isolated repeatedly from single patients during recurrent episodes of Candida infection in a hospital, and their electrophoretic karyotypes were analyzed by pulsed-field gel electrophoresis using CHEF system. When only C. albicans (in 6 patients) or C. glabrata (in 1 patient) were recurrently isolated, their karyotypes from each patient were almost identical to one another, suggesting that they carried single type of the yeast. When multiple species were recovered from single patients (6 cases), the karyotypes of the most frequently recovered yeast species were almost identical with respect to each patient. The electrophoretic karyotype analysis has been proved to be useful for epidemiological studies because the method can tell not only the species identification but also the differences among the strains of the same species.
在一家医院里，念珠菌感染反复发作期间，从单一患者身上反复分离出念珠菌菌株，并使用CHEF系统通过脉冲场凝胶电泳分析其电泳核型。当仅反复分离出白色念珠菌（6例患者）或光滑念珠菌（1例患者）时，每位患者的核型几乎彼此相同，这表明它们携带单一类型的酵母。当从单一患者身上分离出多种念珠菌（6例）时，每位患者中最常分离出的酵母菌种的核型几乎相同。电泳核型分析已被证明对流行病学研究有用，因为该方法不仅可以鉴定菌种，还可以区分同一菌种不同菌株之间的差异。
