Zhao M, Zhang N X, Laissue J A, Zimmermann A
Institut of Pathology, University of Bern, Switzerland.
Virchows Arch. 1994;424(6):613-21. doi: 10.1007/BF00195775.
We analysed p53 protein immunoreactivity in hepatocellular carcinomas (HCCs) and in liver cell dysplasia (LCD) of patients from an area in Northern China, using five anti-p53 protein antibodies recognizing different epitopes of the protein. In HCCs, the overall prevalence of p53 protein immunoreactivity was 78.3%. However, prevalence was strongly influenced by the type of antibody used, ranging from 67.5% for antibody PAb-1801 to only 10.8% for antibodies PAb-421 and DO-7. p53 protein immunoreactivity was not related to type or grade of HCC. In contrast to former reports, p53 protein staining was restricted to nuclei only when using the CM-1 antibody, whereas two other antibodies yielded both, nuclear and cytoplasmic or membrane staining, and no nuclear staining was observed with antibodies PAb-421 and DO-7, the latter two, however, demonstrating cytoplasmic and membrane staining. For LCD, three subtypes were morphologically and karyometrically defined. Nuclei of some LCD cells were p53 immunoreactive, but positivity was restricted to the small cell variant of LCD. Positivity was different for cirrhosis with or without associated HCC, amounting to 18.9% in the former and 39.4% in the latter. Interestingly, p53 protein immunoreactivity also occurred in a set of small hepatocytes not showing the typical feature of LCD and therefore classified as simple regenerating liver cells.
我们使用五种识别该蛋白不同表位的抗p53蛋白抗体，分析了中国北方某地区患者肝细胞癌（HCC）和肝细胞发育异常（LCD）中的p53蛋白免疫反应性。在HCC中，p53蛋白免疫反应性的总体患病率为78.3%。然而，患病率受所用抗体类型的强烈影响，范围从抗体PAb - 1801的67.5%到抗体PAb - 421和DO - 7的仅10.8%。p53蛋白免疫反应性与HCC的类型或分级无关。与以前的报道相反，仅在使用CM - 1抗体时，p53蛋白染色才仅限于细胞核，而另外两种抗体产生细胞核和细胞质或细胞膜染色，并且用抗体PAb - 421和DO - 7未观察到细胞核染色，不过后两者显示出细胞质和细胞膜染色。对于LCD，从形态学和核测量学上定义了三种亚型。一些LCD细胞的细胞核具有p53免疫反应性，但阳性仅限于LCD的小细胞变体。伴有或不伴有相关HCC的肝硬化的阳性率不同，前者为18.9%，后者为39.4%。有趣的是，p53蛋白免疫反应性也出现在一组未显示LCD典型特征、因此被归类为单纯再生肝细胞的小肝细胞中。
