Elkihel L, Soustre I, Karst F, Letourneux Y
Laboratoire de Physicochimie des Substances Naturelles, Université de La Rochelle, France.
FEMS Microbiol Lett. 1994 Jul 1;120(1-2):163-7. doi: 10.1111/j.1574-6968.1994.tb07024.x.
Among a series of aminocholesterol derivatives synthesized, 7-aminocholesterol is the strongest inhibitor of yeast cell growth. Using sterol auxotrophic mutant strains, we showed that this compound inhibits cell proliferation by interfering with ergosterol biosynthesis. The sterol pattern of treated cells revealed that 7-aminocholesterol inhibits delta 8-->delta 7-sterol isomerase and delta 14-sterol reductase as morpholine inhibitors. However, the novel feature of this compound is a strong cytotoxicity to yeast.
在一系列合成的氨基胆固醇衍生物中，7-氨基胆固醇是酵母细胞生长的最强抑制剂。利用甾醇营养缺陷型突变菌株，我们发现该化合物通过干扰麦角固醇的生物合成来抑制细胞增殖。处理后细胞的甾醇模式表明，7-氨基胆固醇作为吗啉类抑制剂可抑制δ8→δ7-甾醇异构酶和δ14-甾醇还原酶。然而，该化合物的新特点是对酵母具有很强的细胞毒性。
