Konishi E, Uehara K
Department of Medical Zoology, Kobe University School of Medicine, Japan.
J Med Entomol. 1994 May;31(3):394-9. doi: 10.1093/jmedent/31.3.394.
A medical service system for testing antigen levels of Dermatophagoides farinae Hughes and D. pteronyssinus (Trouessart) mites in house dust was established to allow physicians to monitor the environment of patients with allergies. Dust samples were collected from a 1-m2 area at different sites for 1 min using a commercial collection apparatus attached to a vacuum cleaner. Extracts from fine dust fractions were processed quantitatively for antigen by a sandwich enzyme-linked immunosorbent assay. Mean antigen levels contained in samples from carpets, tatamis, and futons ranged from 431 to 852 micrograms per unit weight of dust (g). When data were expressed as micrograms per m2, the mean antigen level obtained from carpets (41 micrograms/m2) was significantly higher than that obtained from tatamis (15 micrograms/m2) and futons (14 micrograms/m2). Comparison of 37 paired samples collected from 10 homes at an interval of 1 to 2 mo indicated that high antigen levels of > 50 micrograms/m2 at four sites (three carpets and one tatami) decreased sharply after improvement of hygienic conditions surrounding allergic patients.
建立了一种用于检测室内灰尘中粉尘螨和屋尘螨抗原水平的医疗服务系统，以便医生监测过敏患者的环境。使用连接到吸尘器的商用收集装置，在不同地点从1平方米的区域采集灰尘样本1分钟。通过夹心酶联免疫吸附测定法定量处理细粉尘级分中的提取物以检测抗原。地毯、榻榻米和蒲团样本中所含的平均抗原水平为每单位重量灰尘（克）431至852微克。当数据以每平方米微克表示时，从地毯获得的平均抗原水平（41微克/平方米）显著高于从榻榻米（15微克/平方米）和蒲团（14微克/平方米）获得的水平。对从10个家庭每隔1至2个月收集的37对样本进行比较表明，在改善过敏患者周围的卫生条件后，四个地点（三块地毯和一块榻榻米）高于50微克/平方米的高抗原水平急剧下降。
