Twiggs J T, Gray R L, DeJongh S, Marx J J
Department of Allergy, Marshfield Clinic, WI 54449.
Ann Allergy. 1991 May;66(5):431-5.
The allergens produced by the dust mites of the Dermatophagoides genus are now recognized as one of the major allergens in house dust. Quantification of those allergens helps to evaluate the effectiveness of avoidance measures and specific treatments aimed at the reduction of the mites and their allergens. The present study was undertaken to compare the level of D. farinae allergen collected by a standard vacuum cleaner with a disposable bag and the level of allergen collected by a hand-held vacuum equipped with a special filter. Samples were obtained from the main living area of 18 homes of patients allergic to D. farinae. Using a hand-held vacuum especially equipped with a removable micropore filter, a 2-m2 area of carpet was vacuumed for two minutes and an identical sample was collected from the major upholstered piece of furniture in the room. A third sample was obtained with an upright vacuum fitted with a clean disposable bag. The dust collected was extracted in phosphate buffered saline and resultant extracts were assayed for D. farinae allergen content by microtiter plate RAST inhibition assay. Results from these assays showed a large interhome variability regardless of sampling technique, but intrahome sampling showed remarkable consistency. A linear regression analysis demonstrated all of the comparisons correlated at a probability level of P less than .05. We conclude that dust mite allergen levels collected in a disposable bag vacuum cleaner are reliable indicators of the level of house dust mites present in the homes of patients.
屋尘螨属的尘螨产生的过敏原现已被公认为室内灰尘中的主要过敏原之一。对这些过敏原进行定量有助于评估旨在减少螨虫及其过敏原的回避措施和特异性治疗的效果。本研究旨在比较配备一次性袋子的标准真空吸尘器收集的粉尘螨过敏原水平与配备特殊过滤器的手持式真空吸尘器收集的过敏原水平。样本取自18名对粉尘螨过敏患者家中的主要居住区域。使用特别配备可移动微孔过滤器的手持式真空吸尘器，对2平方米的地毯区域吸尘两分钟，并从房间内主要的软体家具上采集相同的样本。第三个样本是用配备干净一次性袋子的直立式真空吸尘器采集的。将收集到的灰尘在磷酸盐缓冲盐水中提取，所得提取物通过微量滴定板RAST抑制试验检测粉尘螨过敏原含量。这些试验结果显示，无论采用何种采样技术，家庭间的差异都很大，但家庭内采样显示出显著的一致性。线性回归分析表明，所有比较在P值小于0.05的概率水平上具有相关性。我们得出结论，一次性袋子真空吸尘器收集的尘螨过敏原水平是患者家中屋尘螨存在水平的可靠指标。
