Mazur S, Goodhardt M, Feunteun J, de La Roche Saint André C
Laboratoire d'Oncologie Moléculaire, Unité de Recherche Associeé 1158, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, France.
J Virol. 1994 Sep;68(9):5629-37. doi: 10.1128/JVI.68.9.5629-5637.1994.
Hamster polyomavirus (HaPV) causes lymphomas when injected into newborn hamsters. These tumors are virus-free but accumulate large amounts of deleted extrachromosomal viral genomes. In order to identify the major sites of virus replication in animals, we have monitored the HaPV DNA present in different organs at various times after injection. The data demonstrate that viral replication preferentially occurs in lymphoid organs. Lymphoma-associated viral genomes display specific deletions. PCR analysis shows that such viral genomes are the only variants detectable in infected animals, suggesting that they are generated by a specific cellular mechanism. We have tested the possible role of the lymphoid cell-specific V(D)J recombination activity in the generation of these specific variants. Our results indicate that this mechanism is not solely responsible for the viral genome rearrangement, if involved at all.
仓鼠多瘤病毒（HaPV）注射到新生仓鼠体内时会引发淋巴瘤。这些肿瘤不含病毒，但积累了大量缺失的染色体外病毒基因组。为了确定病毒在动物体内的主要复制位点，我们监测了注射后不同时间不同器官中存在的HaPV DNA。数据表明病毒复制优先发生在淋巴器官。与淋巴瘤相关的病毒基因组显示出特定的缺失。PCR分析表明，此类病毒基因组是感染动物中唯一可检测到的变体，这表明它们是由特定的细胞机制产生的。我们测试了淋巴细胞特异性V(D)J重组活性在这些特定变体产生中的可能作用。我们的结果表明，该机制即便参与其中，也并非病毒基因组重排的唯一原因。
