Okada Y, Rahmann H, Hirai H, Terashima A
Department of Physiology, School of Medicine, Kobe University, Japan.
Neurosci Lett. 1994 Apr 11;170(2):269-72. doi: 10.1016/0304-3940(94)90335-2.
To confirm the effect of gangliosides on the facilitation of the induction of long-term potentiation (LTP), slices of hippocampus and superior colliculus from guinea pig were prepared. One group of slices was incubated in the standard medium containing gangliosides (GM1, GD1a, or a mixture of gangliosides from bovine brain, each at a concentration of 70 microM) for 2 h. The other group of slices was incubated in the standard medium only. In both groups of slices, tetanic stimulation induced LTP in a similar manner. In low Ca2+ (1 mM) medium, LTP was not induced in either group of slices. Thus, the present experiments could not confirm previous reports indicating that exogenously applied gangliosides facilitate the induction of LTP.
为了证实神经节苷脂对促进长时程增强（LTP）诱导的作用，制备了豚鼠海马和上丘切片。一组切片在含有神经节苷脂（GM1、GD1a或牛脑来源的神经节苷脂混合物，每种浓度均为70微摩尔）的标准培养基中孵育2小时。另一组切片仅在标准培养基中孵育。在两组切片中，强直刺激以相似的方式诱导LTP。在低钙（1毫摩尔）培养基中，两组切片均未诱导出LTP。因此，本实验无法证实先前报道的外源性应用神经节苷脂可促进LTP诱导的说法。
