Buchanan-Wollaston V
Department of Biological Sciences, Wye College, University of London, Ashford, Kent, United Kingdom.
Plant Physiol. 1994 Jul;105(3):839-46. doi: 10.1104/pp.105.3.839.
cDNA clones representing genes that are expressed during leaf senescence in Brassica napus were identified by differential screening of a cDNA library made from RNA isolated from leaves at different stages of senescence. The expression of these genes at different stages of leaf development was examined by northern blot analysis, and several different patterns of expression were observed. One of the clones, LSC54, represented a gene that is expressed at high levels during leaf senescence. Analysis of this gene indicated strong expression in flowers as well as in senescing leaves. DNA sequence analysis of the LSC54 cDNA indicated a similarity between the deduced amino acid sequence and several metallothionein-like proteins previously identified in plants.
通过对从不同衰老阶段叶片分离的RNA构建的cDNA文库进行差异筛选，鉴定出代表甘蓝型油菜叶片衰老过程中表达基因的cDNA克隆。通过Northern印迹分析检测了这些基因在叶片发育不同阶段的表达情况，并观察到几种不同的表达模式。其中一个克隆LSC54代表一个在叶片衰老期间高水平表达的基因。对该基因的分析表明，它在花和衰老叶片中均有强烈表达。LSC54 cDNA的DNA序列分析表明，其推导的氨基酸序列与先前在植物中鉴定的几种类金属硫蛋白相似。
