甘蓝型油菜叶片衰老过程中表达基因的cDNA克隆分离。一种编码衰老特异性金属硫蛋白样蛋白的基因的鉴定。
Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein.
作者信息
Buchanan-Wollaston V
机构信息
Department of Biological Sciences, Wye College, University of London, Ashford, Kent, United Kingdom.
出版信息
Plant Physiol. 1994 Jul;105(3):839-46. doi: 10.1104/pp.105.3.839.
Abstract
cDNA clones representing genes that are expressed during leaf senescence in Brassica napus were identified by differential screening of a cDNA library made from RNA isolated from leaves at different stages of senescence. The expression of these genes at different stages of leaf development was examined by northern blot analysis, and several different patterns of expression were observed. One of the clones, LSC54, represented a gene that is expressed at high levels during leaf senescence. Analysis of this gene indicated strong expression in flowers as well as in senescing leaves. DNA sequence analysis of the LSC54 cDNA indicated a similarity between the deduced amino acid sequence and several metallothionein-like proteins previously identified in plants.
摘要
通过对从不同衰老阶段叶片分离的RNA构建的cDNA文库进行差异筛选，鉴定出代表甘蓝型油菜叶片衰老过程中表达基因的cDNA克隆。通过Northern印迹分析检测了这些基因在叶片发育不同阶段的表达情况，并观察到几种不同的表达模式。其中一个克隆LSC54代表一个在叶片衰老期间高水平表达的基因。对该基因的分析表明，它在花和衰老叶片中均有强烈表达。LSC54 cDNA的DNA序列分析表明，其推导的氨基酸序列与先前在植物中鉴定的几种类金属硫蛋白相似。
相似文献
1
Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein.甘蓝型油菜叶片衰老过程中表达基因的cDNA克隆分离。一种编码衰老特异性金属硫蛋白样蛋白的基因的鉴定。
Plant Physiol. 1994 Jul;105(3):839-46. doi: 10.1104/pp.105.3.839.
2
Leaf senescence in Brassica napus: cloning of senescence related genes by subtractive hybridisation.甘蓝型油菜叶片衰老：通过消减杂交克隆衰老相关基因
Plant Mol Biol. 1997 Mar;33(5):821-34. doi: 10.1023/a:1005774212410.
3
Leaf senescence in Brassica napus: expression of genes encoding pathogenesis-related proteins.甘蓝型油菜叶片衰老：编码病程相关蛋白的基因表达
Plant Mol Biol. 1996 Feb;30(3):597-609. doi: 10.1007/BF00049334.
4
Classification and expression of a family of cyclin gene homologues in Brassica napus.甘蓝型油菜中一个细胞周期蛋白基因同源物家族的分类与表达
Plant Mol Biol. 1995 Jan;27(2):263-75. doi: 10.1007/BF00020182.
5
Molecular analysis of two Brassica napus genes expressed in the stigma.对两个在柱头中表达的甘蓝型油菜基因的分子分析。
Plant Mol Biol. 1994 Nov;26(4):1217-22. doi: 10.1007/BF00040703.
6
Isolation and characterization of two Brassica napus embryo acyl-ACP thioesterase cDNA clones.两个甘蓝型油菜胚酰基-ACP硫酯酶cDNA克隆的分离与鉴定
Plant Mol Biol. 1993 Nov;23(4):769-78. doi: 10.1007/BF00021532.
7
Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene.来自甘蓝型油菜的两个相关的低温诱导基因与人类肿瘤bbc1（乳腺基本保守）基因同源。
Plant Mol Biol. 1993 Dec;23(6):1211-21. doi: 10.1007/BF00042354.
8
Characterization of three anther-specific genes isolated from Chinese cabbage.从大白菜中分离出的三个花药特异性基因的特征分析
Plant Mol Biol. 1997 Jan;33(1):193-8. doi: 10.1023/a:1005790832221.
9
Characterization of gene repertoires at mature stage of citrus fruits through random sequencing and analysis of redundant metallothionein-like genes expressed during fruit development.通过随机测序和分析果实发育过程中表达的冗余类金属硫蛋白基因来表征柑橘果实成熟阶段的基因库。
Gene. 1998 May 12;211(2):221-7. doi: 10.1016/s0378-1119(98)00138-3.
10
Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing.通过单次部分测序生成甘蓝型油菜随机根cDNA克隆的表达序列标签
Plant Physiol. 1993 Oct;103(2):359-70. doi: 10.1104/pp.103.2.359.
引用本文的文献
1
Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn.动物、植物和细菌金属硫蛋白中不同的锌(II)结合亲和力在生理 pZn 下定义了它们的锌缓冲能力。
Metallomics. 2023 Oct 4;15(10). doi: 10.1093/mtomcs/mfad061.
2
Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought.响应干旱时，甜燕麦生理生化参数和金属硫蛋白表达的变化。
Sci Rep. 2023 Feb 12;13(1):2486. doi: 10.1038/s41598-023-29394-2.
3
Barley metallothionein isoforms, MT2b2 and MT4, differentially respond to photohormones in barley aleurone layer and their recombinant forms show different affinity for binding to zinc and cadmium.大麦金属硫蛋白同工型 MT2b2 和 MT4 对大麦糊粉层的光敏激素有不同的反应，它们的重组形式对锌和镉的结合亲和力也不同。
Biometals. 2023 Feb;36(1):3-18. doi: 10.1007/s10534-022-00452-y. Epub 2022 Oct 30.
4
Cloning and expression of multiple metallothioneins from hybrid poplar.杂种杨树中多种金属硫蛋白的克隆与表达
New Phytol. 2004 Oct;164(1):83-93. doi: 10.1111/j.1469-8137.2004.01168.x.
5
Characterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper.拟南芥金属硫蛋白基因家族的特征：衰老过程中及对铜响应时的组织特异性表达与诱导
New Phytol. 2003 Aug;159(2):369-381. doi: 10.1046/j.1469-8137.2003.00813.x.
6
Overexpression of from moso bamboo promotes leaf senescence and enhances abiotic stress tolerance in .毛竹中[具体物质]的过表达促进叶片衰老并增强[具体对象]对非生物胁迫的耐受性。 （原文中“from moso bamboo”和“in ”处信息不完整）
PeerJ. 2020 Mar 31;8:e8716. doi: 10.7717/peerj.8716. eCollection 2020.
7
Global transcriptome analysis of alfalfa reveals six key biological processes of senescent leaves.苜蓿的全转录组分析揭示了衰老叶片的六个关键生物学过程。
PeerJ. 2020 Jan 21;8:e8426. doi: 10.7717/peerj.8426. eCollection 2020.
8
Cell death patterns in Arabidopsis cells subjected to four physiological stressors indicate multiple signalling pathways and cell cycle phase specificity.拟南芥细胞在遭受四种生理应激源时的细胞死亡模式表明存在多种信号通路和细胞周期阶段特异性。
Protoplasma. 2017 Mar;254(2):635-647. doi: 10.1007/s00709-016-0977-8. Epub 2016 May 18.
9
Physiological Characterization and Comparative Transcriptome Analysis of a Slow-Growing Reduced-Thylakoid Mutant of Chinese Cabbage (Brassica campestris ssp. pekinensis).大白菜（Brassica campestris ssp. pekinensis）一个生长缓慢的类囊体减少突变体的生理特性及比较转录组分析
Front Plant Sci. 2016 Jan 26;7:3. doi: 10.3389/fpls.2016.00003. eCollection 2016.
10
Deep sequencing of the Mexican avocado transcriptome, an ancient angiosperm with a high content of fatty acids.对墨西哥鳄梨转录组进行深度测序，墨西哥鳄梨是一种脂肪酸含量高的古老被子植物。
BMC Genomics. 2015 Aug 13;16(1):599. doi: 10.1186/s12864-015-1775-y.
本文引用的文献
1
Induction of Malate Synthase Gene Expression in Senescent and Detached Organs of Cucumber.黄瓜衰老及离体器官中苹果酸合酶基因表达的诱导
Plant Cell. 1992 Mar;4(3):349-357. doi: 10.1105/tpc.4.3.349.
2
Expression, organisation and structure of the genes encoding the waxy protein (granule-bound starch synthase) in wheat.小麦中编码蜡质蛋白（颗粒结合型淀粉合酶）的基因的表达、组织与结构
Plant Mol Biol. 1993 Apr;22(1):67-82. doi: 10.1007/BF00038996.
3
Metallothionein protects DNA from oxidative damage.金属硫蛋白可保护DNA免受氧化损伤。
Biochem J. 1993 Apr 1;291 ( Pt 1)(Pt 1):193-8. doi: 10.1042/bj2910193.
4
Plant metallothioneins.植物金属硫蛋白
Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):1-10. doi: 10.1042/bj2950001.
5
Structure and developmental regulation of a wheat gene encoding the major chlorophyll a/b-binding polypeptide.一个编码主要叶绿素a/b结合多肽的小麦基因的结构与发育调控
Mol Cell Biol. 1985 Jun;5(6):1370-8. doi: 10.1128/mcb.5.6.1370-1378.1985.
6
A gene from pea (Pisum sativum L.) with homology to metallothionein genes.来自豌豆（Pisum sativum L.）的一个与金属硫蛋白基因具有同源性的基因。
FEBS Lett. 1990 Mar 12;262(1):29-32. doi: 10.1016/0014-5793(90)80145-9.
7
Eukaryotic start and stop translation sites.真核生物翻译起始和终止位点。
Nucleic Acids Res. 1991 Jun 25;19(12):3185-92. doi: 10.1093/nar/19.12.3185.
8
An iron deficiency-specific cDNA from barley roots having two homologous cysteine-rich MT domains.一种来自大麦根的缺铁特异性cDNA，具有两个富含半胱氨酸的同源金属硫蛋白结构域。
Plant Mol Biol. 1991 Sep;17(3):531-3. doi: 10.1007/BF00040651.
Zotero 插件