类黄酮可以保护玉米DNA免受紫外线辐射损伤的诱导。
Flavonoids can protect maize DNA from the induction of ultraviolet radiation damage.
作者信息
Stapleton A E, Walbot V
机构信息
Department of Biological Sciences, Stanford University, California 94305-5020.
出版信息
Plant Physiol. 1994 Jul;105(3):881-9. doi: 10.1104/pp.105.3.881.
Abstract
Diverse flavonoid compounds are widely distributed in angiosperm families. Flavonoids absorb radiation in the ultraviolet (UV) region of the spectrum, and it has been proposed that these compounds function as UV filters. We demonstrate that the DNA in Zea mays plants that contain flavonoids (primarily anthocyanins) is protected from the induction of damage caused by UV radiation relative to the DNA in plants that are genetically deficient in these compounds. DNA damage was measured with a sensitive and simple assay using individual monoclonal antibodies, one specific for cyclobutane pyrimidine dimer damage and the other specific for pyrimidine(6,4)pyrimidone damage.
摘要
多种黄酮类化合物广泛分布于被子植物科中。黄酮类化合物在光谱的紫外线（UV）区域吸收辐射，有人提出这些化合物起到紫外线滤光剂的作用。我们证明，相对于那些在这些化合物上存在基因缺陷的植物中的DNA，含有黄酮类化合物（主要是花青素）的玉米植株中的DNA受到保护，免受紫外线辐射诱导的损伤。使用灵敏且简单的检测方法来测量DNA损伤，该方法使用单个单克隆抗体，一种对环丁烷嘧啶二聚体损伤具有特异性，另一种对嘧啶（6,4）嘧啶酮损伤具有特异性。
相似文献
1
Flavonoids can protect maize DNA from the induction of ultraviolet radiation damage.类黄酮可以保护玉米DNA免受紫外线辐射损伤的诱导。
Plant Physiol. 1994 Jul;105(3):881-9. doi: 10.1104/pp.105.3.881.
2
Measurement of UV radiation-induced DNA damage using specific antibodies.使用特异性抗体测量紫外线辐射诱导的DNA损伤。
Methods Mol Biol. 1999;113:157-63. doi: 10.1385/1-59259-675-4:157.
3
Kinetics of pyrimidine(6-4)pyrimidone photoproduct repair in Escherichia coli.大肠杆菌中嘧啶（6-4）嘧啶酮光产物修复的动力学
J Bacteriol. 1996 Mar;178(5):1347-50. doi: 10.1128/jb.178.5.1347-1350.1996.
4
Regulation of plant MSH2 and MSH6 genes in the UV-B-induced DNA damage response.UV-B 诱导的 DNA 损伤响应中植物 MSH2 和 MSH6 基因的调控。
J Exp Bot. 2011 May;62(8):2925-37. doi: 10.1093/jxb/err001. Epub 2011 Feb 9.
5
6
Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in Arabidopsis mutants altered in phenolic metabolism.紫外线B辐射影响拟南芥酚类代谢改变突变体中光系统II反应中心异二聚体的光介导周转。
Plant Physiol. 2000 Nov;124(3):1275-84. doi: 10.1104/pp.124.3.1275.
7
Pyrimidine dimers in DNA initiate systemic immunosuppression in UV-irradiated mice.DNA中的嘧啶二聚体在紫外线照射的小鼠中引发全身免疫抑制。
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7516-20. doi: 10.1073/pnas.89.16.7516.
8
Response of purely symbiotic and NO3-fed nodulated plants of Lupinus luteus and Vicia atropurpurea to ultraviolet-B radiation.羽扇豆和紫花野豌豆的纯共生及供应硝酸盐结瘤植株对紫外线B辐射的响应
J Exp Bot. 2003 Jul;54(388):1771-84. doi: 10.1093/jxb/erg190.
9
Gene expression profiling in response to ultraviolet radiation in maize genotypes with varying flavonoid content.不同类黄酮含量玉米基因型对紫外线辐射响应的基因表达谱分析
Plant Physiol. 2003 Aug;132(4):1739-54. doi: 10.1104/pp.103.022871.
10
Detecting ultraviolet damage in single DNA molecules by atomic force microscopy.通过原子力显微镜检测单个DNA分子中的紫外线损伤。
Biophys J. 2007 Sep 1;93(5):1758-67. doi: 10.1529/biophysj.107.108209. Epub 2007 May 4.
引用本文的文献
1
Transcriptomic and Metabolomic Insights into Plant Hormone Modulation and Secondary Metabolite Accumulation in Basil Under Far-Red and Ultraviolet-A Light.转录组学和代谢组学揭示远红光和紫外线-A光对罗勒中植物激素调节和次生代谢产物积累的影响
Int J Mol Sci. 2025 Apr 16;26(8):3758. doi: 10.3390/ijms26083758.
2
Integrated metabolomics and transcriptomics analysis comprehensively revealed the underlying metabolite mechanism difference between Male and Female Eucommia ulmoides Oliver.整合代谢组学和转录组学分析全面揭示了杜仲雌雄植株之间潜在的代谢物机制差异。
BMC Plant Biol. 2025 May 2;25(1):573. doi: 10.1186/s12870-025-06575-x.
3
Roles of ROS in physiological, microbial and metabolomic alterations of fresh-cut sugarcane under red and blue light irradiation.活性氧在红光和蓝光照射下鲜切甘蔗生理、微生物和代谢组学变化中的作用
Food Chem X. 2025 Mar 4;26:102344. doi: 10.1016/j.fochx.2025.102344. eCollection 2025 Feb.
4
Floral traits underlying mating system differentiation in the wind-pollinated sister species and .风媒传粉姊妹种[物种名称1]和[物种名称2]中交配系统分化所基于的花部性状。
AoB Plants. 2024 Dec 31;17(1):plae073. doi: 10.1093/aobpla/plae073. eCollection 2025 Jan.
5
Comparative transcriptome analysis of differentially expressed genes and pathways in male and female flowers of Fraxinus mandshurica.比较转录组分析白蜡树雌雄花中差异表达基因和通路。
PLoS One. 2024 Sep 12;19(9):e0308013. doi: 10.1371/journal.pone.0308013. eCollection 2024.
6
Poaceae plants transfer cyclobutane pyrimidine dimer photolyase to chloroplasts for ultraviolet-B resistance.禾本科植物将环丁烷嘧啶二聚体光解酶转移到叶绿体中以抵抗紫外线-B。
Plant Physiol. 2024 Apr 30;195(1):326-342. doi: 10.1093/plphys/kiae060.
7
MYB24 orchestrates terpene and flavonol metabolism as light responses to anthocyanin depletion in variegated grape berries.MYB24 作为光响应调控萜烯和类黄酮代谢，以应对葡萄果实杂色中花青素的耗竭。
Plant Cell. 2023 Nov 30;35(12):4238-4265. doi: 10.1093/plcell/koad228.
8
A Small Molecule That Promotes Cellular Senescence Prevents Fibrogenesis and Tumorigenesis.一种促进细胞衰老的小分子可预防纤维化和肿瘤发生。
Int J Mol Sci. 2022 Jun 20;23(12):6852. doi: 10.3390/ijms23126852.
9
Drought and UV Radiation Stress Tolerance in Rice Is Improved by Overaccumulation of Non-Enzymatic Antioxidant Flavonoids.非酶抗氧化类黄酮的过量积累提高了水稻对干旱和紫外线辐射胁迫的耐受性。
Antioxidants (Basel). 2022 May 6;11(5):917. doi: 10.3390/antiox11050917.
10
Plant Secondary Metabolites Produced in Response to Abiotic Stresses Has Potential Application in Pharmaceutical Product Development.植物次生代谢产物对非生物胁迫的响应具有在药物产品开发中的潜在应用。
Molecules. 2022 Jan 5;27(1):313. doi: 10.3390/molecules27010313.
本文引用的文献
1
UV-B-Inducible and Temperature-Sensitive Photoreactivation of Cyclobutane Pyrimidine Dimers in Arabidopsis thaliana.UV-B 诱导和温度敏感的拟南芥中环丁烷嘧啶二聚体的光修复。
Plant Physiol. 1991 Feb;95(2):536-43. doi: 10.1104/pp.95.2.536.
2
Effects of Ultraviolet-B Irradiance on Soybean : V. The Dependence of Plant Sensitivity on the Photosynthetic Photon Flux Density during and after Leaf Expansion.紫外线B辐照度对大豆的影响：V. 叶片扩展期间及之后植物敏感性对光合光子通量密度的依赖性。
Plant Physiol. 1984 Mar;74(3):475-80. doi: 10.1104/pp.74.3.475.
3
Ultraviolet Radiation and Plants: Burning Questions.紫外线辐射与植物：亟待解决的重要问题
Plant Cell. 1992 Nov;4(11):1353-1358. doi: 10.1105/tpc.4.11.1353.
4
Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation.拟南芥类黄酮突变体对UV-B辐射高度敏感。
Plant Cell. 1993 Feb;5(2):171-179. doi: 10.1105/tpc.5.2.171.
5
The Role of Flavonol Glycosides and Carotenoids in Protecting Soybean from Ultraviolet-B Damage.黄酮醇糖苷和类胡萝卜素在保护大豆免受紫外线B损伤中的作用。
Plant Physiol. 1993 Nov;103(3):741-752. doi: 10.1104/pp.103.3.741.
6
Erythema and melanogenesis action spectra of normal human skin.正常人体皮肤的红斑和黑素生成作用光谱。
Photochem Photobiol. 1982 Aug;36(2):187-91. doi: 10.1111/j.1751-1097.1982.tb04362.x.
7
Quantitation of radiation-, chemical-, or enzyme-induced single strand breaks in nonradioactive DNA by alkaline gel electrophoresis: application to pyrimidine dimers.通过碱性凝胶电泳对非放射性DNA中辐射、化学或酶诱导的单链断裂进行定量分析：应用于嘧啶二聚体。
Anal Biochem. 1986 Oct;158(1):119-29. doi: 10.1016/0003-2697(86)90599-3.
8
Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris.菜豆中编码植物防御酶查尔酮合酶的基因家族的组织与差异激活
Mol Gen Genet. 1987 Dec;210(2):219-33. doi: 10.1007/BF00325687.
9
Regulation of Mu element copy number in maize lines with an active or inactive Mutator transposable element system.具有活跃或不活跃Mutator转座元件系统的玉米品系中Mu元件拷贝数的调控
Mol Gen Genet. 1988 Jan;211(1):27-34. doi: 10.1007/BF00338389.
10
The biology of the (6-4) photoproduct.
Photochem Photobiol. 1989 Jun;49(6):805-19. doi: 10.1111/j.1751-1097.1989.tb05578.x.
Zotero 插件