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2
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本文引用的文献

1
Localization of ATP Sulfurylase and O-Acetylserine(thiol)lyase in Spinach Leaves.在菠菜叶片中定位三磷酸腺苷硫酸化酶和 O-乙酰丝氨酸(巯基)裂解酶。
Plant Physiol. 1990 Nov;94(3):1345-52. doi: 10.1104/pp.94.3.1345.
2
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
3
Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts.两种核编码叶绿体蛋白的前体在被导入叶绿体之前会与外被膜结合。
J Biol Chem. 1985 Mar 25;260(6):3691-6.
4
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。
Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.
5
Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal.苜蓿根瘤菌的共生宿主特异性由一种硫酸化和酰化的氨基葡萄糖寡糖信号决定。
Nature. 1990 Apr 19;344(6268):781-4. doi: 10.1038/344781a0.
6
A conserved cleavage-site motif in chloroplast transit peptides.叶绿体转运肽中的保守切割位点基序。
FEBS Lett. 1990 Feb 26;261(2):455-8. doi: 10.1016/0014-5793(90)80614-o.
7
ATP sulfurylase from trophosome tissue of Riftia pachyptila (hydrothermal vent tube worm).来自巨型管虫(热液喷口管虫)营养体组织的ATP硫酸化酶。
Arch Biochem Biophys. 1991 Oct;290(1):66-78. doi: 10.1016/0003-9861(91)90592-7.
8
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.λ YES:一种用于通过酵母和大肠杆菌突变互补来分离基因的多功能cDNA表达载体。
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731-5. doi: 10.1073/pnas.88.5.1731.

通过在酿酒酵母中进行功能表达克隆拟南芥中编码ATP硫酸化酶的cDNA。

Cloning of a cDNA encoding ATP sulfurylase from Arabidopsis thaliana by functional expression in Saccharomyces cerevisiae.

作者信息

Leustek T, Murillo M, Cervantes M

机构信息

Center for Agricultural Molecular Biology, Rutgers University, New Brunswick, New Jersey 08903.

出版信息

Plant Physiol. 1994 Jul;105(3):897-902. doi: 10.1104/pp.105.3.897.

DOI:10.1104/pp.105.3.897
PMID:8058839
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC160738/
Abstract

ATP sulfurylase, the first enzyme in the sulfate assimilation pathway of plants, catalyzes the formation of adenosine phosphosulfate from ATP and sulfate. Here we report the cloning of a cDNA encoding ATP sulfurylase (APS1) from Arabidopsis thaliana. APS1 was isolated by its ability to alleviate the methionine requirement of an ATP sulfurylase mutant strain of Saccharomyces cerevisiae (yeast). Expression of APS1 correlated with the presence of ATP sulfurylase enzyme activity in cell extracts. APS1 is a 1748-bp cDNA with an open reading frame predicted to encode a 463-amino acid, 51,372-D protein. The predicted amino acid sequence of APS1 is similar to ATP sulfurylase of S. cerevisiae, with which it is 25% identical. Two lines of evidence indicate that APS1 encodes a chloroplast form of ATP sulfurylase. Its predicted amino-terminal sequence resembles a chloroplast transit peptide; and the APS1 polypeptide, synthesized in vitro, is capable of entering isolated intact chloroplasts. Several genomic DNA fragments that hybridize with the APS1 probe were identified. The APS1 cDNA hybridizes to three species of mRNA in leaves (1.85, 1.60, and 1.20 kb) and to a single species of mRNA in roots (1.85 kb).

摘要

ATP硫酸化酶是植物硫酸盐同化途径中的首个酶,催化由ATP和硫酸盐形成腺苷磷酸硫酸。在此,我们报道了从拟南芥中克隆编码ATP硫酸化酶(APS1)的cDNA。通过其缓解酿酒酵母(酵母)ATP硫酸化酶突变株对甲硫氨酸需求的能力分离出APS1。APS1的表达与细胞提取物中ATP硫酸化酶活性的存在相关。APS1是一个1748bp的cDNA,其开放阅读框预测编码一个463个氨基酸、51372道尔顿的蛋白质。APS1预测的氨基酸序列与酿酒酵母的ATP硫酸化酶相似,二者有25%的同源性。有两条证据表明APS1编码叶绿体形式的ATP硫酸化酶。其预测的氨基末端序列类似于叶绿体转运肽;并且体外合成的APS1多肽能够进入分离的完整叶绿体。鉴定出了几个与APS1探针杂交的基因组DNA片段。APS1 cDNA与叶片中的三种mRNA(1.85、1.60和1.20kb)以及根中的一种mRNA(1.85kb)杂交。