Millis R M, Barber J D, Anderson W A, Dehkordi O, Toussaint R M, Ertugrul L
Department of Physiology and Biophysics, College of Medicine, Howard University, Washington, DC 20059.
Ren Fail. 1994;16(3):325-35. doi: 10.3109/08860229409044873.
In a timed study over 75 min, divided into 5 15-min periods, experimental perfusions of isolated rat kidneys using Krebs-Ringer bicarbonate (KRB) containing 3.5% (uncrosslinked) stroma-free hemoglobin (SFH) plus 3.5% of the perfluorochemical Fluosol-DA were found to excrete only 13% as much total hemoglobin (Hb) as control perfusions using KRB containing 7% SFH alone (controls). The glomerular filtration rate was the same for experimentals and controls in 3 periods, and slightly higher in the experimentals in 2 periods. However, urine flow was found to be significantly decreased in all 5 periods for the experimentals relative to the controls. These effects can be explained by reabsorption of glomerular filtrate containing Hb by endocytosis of renal tubular cells.
在一项为期75分钟的定时研究中，该研究分为5个15分钟的时间段，发现使用含有3.5%（未交联）无基质血红蛋白（SFH）加3.5%全氟化合物氟碳乳剂（Fluosol-DA）的 Krebs-Ringer 碳酸氢盐（KRB）对离体大鼠肾脏进行实验灌注时，其排出的总血红蛋白（Hb）仅为单独使用含有7% SFH的KRB进行对照灌注（对照组）时的13%。在3个时间段内，实验组和对照组的肾小球滤过率相同，在2个时间段内实验组的肾小球滤过率略高。然而，相对于对照组，在所有5个时间段内均发现实验组的尿流量显著降低。这些影响可以通过肾小管细胞内吞作用对含有Hb的肾小球滤液的重吸收来解释。
