Millis R M, Barber J D, Anderson W A, Dehkordi O
Department of Physiology, College of Medicine, Howard University Washington, DC 20059, USA.
Ren Fail. 1995 Nov;17(6):675-87. doi: 10.3109/08860229509037635.
Experimental perfusions of isolated rat kidneys were performed with flow rates adjusted to produce comparable glomerular filtration rates (GFR) in control and experimental groups. The experimental perfusate, consisting of Krebs-Ringer bicarbonate (KRB) containing 3.5% (uncrosslinked) stroma-free hemoglobin (SFH) plus 3.5% of the perfluorochemical (PFC) Fluosol-DA, was found to produce only 48% as much urine as the control perfusate consisting of KRB containing 7% dextran (without either SFH or PFC). Perfusion with the experimental SFH/PFC mixture was associated with mean fractional reabsorptions of sodium 3.3% greater and of potassium 5.1% lesser than perfusion with the control KRB (with dextran) solution (p < .05). The SFH/PFC mixture was localized histochemically to the capillaries and urinary spaces of glomeruli; and to the apical microvilli, basal laminae, and intracytoplasmic vacuoles of proximal renal tubular cells. Since the glomerular filtration rate was not a factor, decremental urine production appears to be associated with increased reabsorption of sodium, excretion of potassium, and proximal tubular uptake of the experimental SFH/PFC mixture by endocytosis.
对分离的大鼠肾脏进行实验灌注，调整流速以使对照组和实验组的肾小球滤过率（GFR）具有可比性。实验灌注液由含有3.5%（未交联）无基质血红蛋白（SFH）加3.5%全氟化合物（PFC）氟碳乳剂-DA的 Krebs-Ringer 碳酸氢盐（KRB）组成，结果发现其产生的尿量仅为含有7%右旋糖酐（不含SFH或PFC）的对照灌注液的48%。与对照KRB（含右旋糖酐）溶液灌注相比，用实验性SFH/PFC混合物灌注时，钠的平均重吸收分数高3.3%，钾的平均重吸收分数低5.1%（p < .05）。SFH/PFC混合物通过组织化学方法定位在肾小球的毛细血管和尿腔中；以及近端肾小管细胞的顶端微绒毛、基膜和胞质内空泡中。由于肾小球滤过率不是一个因素，尿量减少似乎与钠重吸收增加、钾排泄增加以及近端肾小管通过内吞作用摄取实验性SFH/PFC混合物有关。
