Oosterlynck D J, Cornillie F J, Waer M, Vandeputte M, Koninckx P R
Rega Institute for Medical Research, Leuven, Belgium.
Fertil Steril. 1991 Jul;56(1):45-51. doi: 10.1016/s0015-0282(16)54414-8.
The role of natural killer (NK) cells in the decreased cellular immunity of women with endometriosis was investigated.
DESIGN, SETTING, PATIENTS: Thirty-four women were investigated prospectively before a CO2-laser laparoscopy for infertility and/or pain at the University Hospital Gasthuisberg. Endometriosis was scored blindly.
The cytotoxicity, directed against the endometrium, was mediated by NK cells because this cytotoxicity could be removed by treating the effector cells with the NK-specific anti-Leu-11b monoclonal antibody. Consequently, we evaluated prospectively in those women the lymphocyte-mediated cytotoxicity toward NK sensitive (K562-assay) and autologous endometrial target cells.
The NK activity (K562-assay) and the cytotoxicity against autologous endometrial cells were similarly decreased in women with endometriosis and correlated with the severity of the disease. Using heterologous effector cells, the decreased chromium release in women with endometriosis was less pronounced but still present.
The decreased cytotoxicity to endometrial cells in women with endometriosis is mainly because of a defect in NK activity but is also partially because of a resistance of the endometrium to NK cytotoxicity.
研究自然杀伤（NK）细胞在子宫内膜异位症患者细胞免疫功能降低中的作用。
设计、地点、患者：前瞻性研究了34名在加斯豪斯贝格大学医院因不孕和/或疼痛接受二氧化碳激光腹腔镜检查的女性。对子宫内膜异位症进行盲法评分。
针对子宫内膜的细胞毒性由NK细胞介导，因为用NK特异性抗Leu-11b单克隆抗体处理效应细胞可消除这种细胞毒性。因此，我们前瞻性地评估了这些女性对NK敏感（K562检测法）和自体子宫内膜靶细胞的淋巴细胞介导的细胞毒性。
子宫内膜异位症患者的NK活性（K562检测法）和对自体子宫内膜细胞的细胞毒性同样降低，且与疾病严重程度相关。使用异源效应细胞时，子宫内膜异位症患者铬释放减少的情况不太明显，但仍然存在。
子宫内膜异位症患者对子宫内膜细胞的细胞毒性降低主要是由于NK活性缺陷，但也部分归因于子宫内膜对NK细胞毒性的抵抗。
