Eppley B L, Summerlin D J, Sadove A M
Division of Plastic and Reconstructive Surgery, Indiana University Medical Center, Indianapolis 46202-5200.
Ann Plast Surg. 1994 May;32(5):463-8. doi: 10.1097/00000637-199405000-00004.
Based on previous experimental connective tissue work in cutaneous wound healing, cell culture, and fat transplantation, the use of positively charged dextran beads for subcutaneous and dermal augmentation was evaluated. When combined with a biocompatible liquid medium, the material easily flowed through small-gauge needles. When injected beneath the facial skin in rats, a profound macrophage response was initially seen at 30 days accompanied by fibroblast proliferation and new collagen formation. By 1 year, a quiescent noninflammatory cellular response with extensive intermaterial collagen deposition was evident. No adverse reactions were seen in the contralateral facial sites, which were injected with only the liquid medium. These preliminary findings suggest good tissue compatibility of this composite biomaterial and provide further evidence of the significant regulatory role of the macrophage in the tissue response to implanted biomaterials.
基于先前在皮肤伤口愈合、细胞培养和脂肪移植方面的结缔组织实验工作，对带正电荷的葡聚糖珠用于皮下和真皮填充进行了评估。当与生物相容性液体介质混合时，该材料很容易通过小口径针头。当注射到大鼠面部皮肤下时，最初在30天时可见强烈的巨噬细胞反应，伴有成纤维细胞增殖和新胶原形成。到1年时，出现了静止的非炎症性细胞反应，材料间有大量胶原沉积。在仅注射液体介质的对侧面部部位未观察到不良反应。这些初步发现表明这种复合生物材料具有良好的组织相容性，并进一步证明了巨噬细胞在组织对植入生物材料的反应中起重要调节作用。
