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白细胞介素-1对兔软骨细胞培养物中碱性磷酸酶、X型胶原蛋白和1,25-二羟维生素D3受体合成及基质钙化的影响。

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

作者信息

Kato Y, Nakashima K, Iwamoto M, Murakami H, Hiranuma H, Koike T, Suzuki F, Fuchihata H, Ikehara Y, Noshiro M

机构信息

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

出版信息

J Clin Invest. 1993 Nov;92(5):2323-30. doi: 10.1172/JCI116836.

DOI:10.1172/JCI116836
PMID:8227347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288413/
Abstract

The effect of IL-1 on expression of the mineralization-related phenotype by chondrocytes was examined. In cultures of rabbit growth plate chondrocytes, IL-1 beta at 0.1 ng/ml caused 95% decreases in alkaline phosphatase activity, alkaline phosphatase mRNA levels, the incorporation of 45Ca into insoluble material, and the calcium content during the hypertrophic stage. These effects of IL-1 beta were dose-dependent and were observed in 24-48 h. Furthermore, IL-1 beta suppressed increase in cell size and the syntheses of 1,25-dihydroxyvitamin D3 receptor and type X collagen, other markers of hypertrophy, but had little effect on the synthesis of total protein including type II collagen. The inhibition of calcification was observed only when chondrocytes were exposed to IL-1 before the onset of calcification: IL-1 treatment from the mineralization stage had a marginal effect on 45Ca incorporation into insoluble material. These results suggest that IL-1 inhibits chondrocyte hypertrophy and the onset of calcification in ossifying cartilage.

摘要

研究了白细胞介素-1（IL-1）对软骨细胞矿化相关表型表达的影响。在兔生长板软骨细胞培养物中，0.1 ng/ml的IL-1β导致肥大期碱性磷酸酶活性、碱性磷酸酶mRNA水平、45Ca掺入不溶性物质以及钙含量降低95%。IL-1β的这些作用具有剂量依赖性，在24 - 48小时内可观察到。此外，IL-1β抑制细胞大小增加以及肥大的其他标志物1,25 - 二羟基维生素D3受体和X型胶原的合成，但对包括II型胶原在内的总蛋白合成影响较小。仅当软骨细胞在矿化开始前暴露于IL-1时才观察到钙化抑制：从矿化阶段开始进行IL-1处理对45Ca掺入不溶性物质的影响很小。这些结果表明，IL-1抑制骨化软骨中软骨细胞的肥大和矿化的开始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/98cce9a85392/jcinvest00043-0249-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/bf446e64140c/jcinvest00043-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/b51ed20d48f6/jcinvest00043-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/e44020fde5fd/jcinvest00043-0248-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/76afe142d88c/jcinvest00043-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/e4b3933702c9/jcinvest00043-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/98cce9a85392/jcinvest00043-0249-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/bf446e64140c/jcinvest00043-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/b51ed20d48f6/jcinvest00043-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/e44020fde5fd/jcinvest00043-0248-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/76afe142d88c/jcinvest00043-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/e4b3933702c9/jcinvest00043-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2992/288413/98cce9a85392/jcinvest00043-0249-c.jpg

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本文引用的文献

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