Bundgaard J R, Sengeløv H, Borregaard N, Kjeldsen L
Department of Clinical Biochemistry, Rigshospitalet, University Hospital, Copenhagen, Denmark.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1468-75. doi: 10.1006/bbrc.1994.2096.
NGAL, a protein recently isolated from human neutrophils, is a novel member of the lipocalins. NGAL binds a derivative of the bacterial chemotactic peptide formylmethionyl-leucyl-phenylalanine and may have important immunomodulatory functions. We here report the cloning of a cDNA for NGAL covering a 63 bp 5' untranslated region and the coding region of 591 bp. The cDNA encodes a protein of 197 amino acids, with a 19 amino acid leader sequence and a mature protein of 178 amino acids. Alignment of the cDNA sequence of NGAL to the rat analogue, alpha 2-microglobulin related protein, demonstrates a very high degree of conservation of this lipocalin. Northern blotting of a variety of tissues revealed that NGAL is mainly expressed in myeloid cells, where a signal of approximately 850 bp is observed. A faint signal was observed in fetal and adult human lung tissue. The molecular cloning of the NGAL cDNA allowed the recombinant production of NGAL in E. coli.
NGAL是一种最近从人中性粒细胞中分离出来的蛋白质，是脂质运载蛋白家族的一个新成员。NGAL能结合细菌趋化肽甲酰甲硫氨酰-亮氨酰-苯丙氨酸的一种衍生物，可能具有重要的免疫调节功能。我们在此报告NGAL的cDNA克隆，其包含一个63bp的5'非翻译区和591bp的编码区。该cDNA编码一个由197个氨基酸组成的蛋白质，带有一个19个氨基酸的前导序列和一个由178个氨基酸组成的成熟蛋白。NGAL的cDNA序列与大鼠类似物α2-微球蛋白相关蛋白的比对显示，这种脂质运载蛋白具有高度的保守性。对多种组织进行的Northern印迹分析表明，NGAL主要在髓样细胞中表达，在那里观察到一个约850bp的信号。在胎儿和成人的肺组织中观察到一个微弱的信号。NGAL cDNA的分子克隆使得能够在大肠杆菌中重组生产NGAL。
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