Cowland J B, Borregaard N
Granulocyte Research Laboratory, Finsen Center, Department of Hematology, Rigshospitalet, Copenhagen University Hospital, Denmark.
Genomics. 1997 Oct 1;45(1):17-23. doi: 10.1006/geno.1997.4896.
Neutrophil gelatinase-associated lipocalin (NGAL) is a 25-kDa lipocalin first identified as a protein stored in specific granules of the human neutrophil. The protein is believed to bind small lipophilic substances such as bacterial derived formylpeptides and lipopolysaccharides (LPS) and might function as a modulator of inflammation. To characterize the regulation of NGAL further, we have cloned and sequenced a 5869-bp region of the NGAL gene including 1695 bp of the 5' nontranscribed region and a 3696-bp coding region encompassing seven exons and six introns. The transcriptional start sites were identified by an RNase protection assay. The NGAL gene is highly homologous to the mouse gene 24p3. NGAL was expressed in bone marrow and in tissues that are prone to exposure to microorganisms. Potential cis-acting elements were identified in the promoter region of the NGAL gene by computer analysis and include binding sites for CTF/CBP, the hematopoietic transcription factors GATA-1 and PU.1, and the LPS-inducible factor NF-kappa B.
中性粒细胞明胶酶相关脂质运载蛋白（NGAL）是一种25 kDa的脂质运载蛋白，最初被鉴定为一种储存在人中性粒细胞特定颗粒中的蛋白质。据信该蛋白可结合小的亲脂性物质，如细菌衍生的甲酰肽和脂多糖（LPS），并可能作为炎症调节剂发挥作用。为了进一步表征NGAL的调控机制，我们克隆并测序了NGAL基因的一个5869 bp区域，包括1695 bp的5'非转录区域和一个3696 bp的编码区域，该编码区域包含7个外显子和6个内含子。通过核糖核酸酶保护试验确定了转录起始位点。NGAL基因与小鼠基因24p3高度同源。NGAL在骨髓和易于接触微生物的组织中表达。通过计算机分析在NGAL基因的启动子区域鉴定出潜在的顺式作用元件，包括CTF/CBP、造血转录因子GATA-1和PU.1以及LPS诱导因子NF-κB的结合位点。
