Fuentealba I C, Bratton G R
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
Anal Cell Pathol. 1994 May;6(4):345-58.
The purpose of this study was to help characterize the pathway of copper in the liver, kidney and duodenum during copper loading and unloading in the rat. Male Wistar rats were allocated randomly into four groups: Group A (control) was composed of 16 animals fed a normal rodent diet. Group B had 16 animals fed a high copper diet (1500 ppm copper). Four rats from each group were killed at 1, 5, 10 and 15 weeks. Group C had 4 animals fed the high copper diet for 5 weeks and normal diet for 5 weeks. Group D consisted of 4 animals fed the high copper diet for 5 weeks, normal diet for 5 weeks, followed by 5 weeks of high copper diet. At termination of each experimental period liver, kidney and duodenum were collected for histochemistry and copper analysis by atomic absorption spectrophotometry. Hepatic copper concentration in Group B rose to 726 +/- 170 micrograms/g after 5 weeks; renal and duodenal copper levels were 285 +/- 14 micrograms/g and 134 +/- 49 micrograms/g, respectively. A significant (P < 0.005) decrease in copper concentration was observed after 15 weeks in all three organs. Duodenal copper concentration in group C was similar to control rats. Changes in copper tissue distribution and efficient unloading were demonstrated in all copper-loaded groups in the three organs studied.
本研究的目的是帮助描绘大鼠在铜负荷和卸载过程中肝脏、肾脏和十二指肠内铜的代谢途径。雄性Wistar大鼠被随机分为四组：A组（对照组）由16只喂食正常啮齿动物饲料的动物组成。B组有16只动物喂食高铜饲料（含1500 ppm铜）。每组中的4只大鼠在1、5、10和15周时被处死。C组有4只动物先喂食高铜饲料5周，再喂食正常饲料5周。D组由4只动物组成，先喂食高铜饲料5周，再喂食正常饲料5周，然后再喂食5周高铜饲料。在每个实验阶段结束时，收集肝脏、肾脏和十二指肠用于组织化学分析以及通过原子吸收分光光度法进行铜分析。B组肝脏铜浓度在5周后升至726±170微克/克；肾脏和十二指肠铜水平分别为285±14微克/克和134±49微克/克。在15周后，所有三个器官中的铜浓度均出现显著下降（P<0.005）。C组十二指肠铜浓度与对照大鼠相似。在所研究的三个器官中，所有铜负荷组均显示出铜组织分布的变化和有效的卸载情况。
