Evering W E, Haywood S, Elmes M E, Jasani B, Trafford J
Department of Veterinary Pathology, University of Liverpool, U.K.
J Pathol. 1990 Apr;160(4):305-12. doi: 10.1002/path.1711600406.
Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metallothionein (MT) have recently been employed for the differential diagnosis of copper-associated diseases. This study compares histochemical with immunocytochemical methods for the assessment of copper status. Male rats were fed a high copper (1 g/kg) diet for 16 weeks and killed sequentially during this period. The livers and kidneys were analysed for copper and zinc (atomic absorption spectrophotometry), and sections were stained with rubeanic acid and rhodanine for copper and for immunoreactive MT using the DNP localization system. Immunoreactive stains for MT corresponded better with copper content than histochemical stains and were more sensitive, albeit less selective, indicators of copper accumulation. Moreover, major differences in intracellular staining were apparent between the two methods, attributed to differences in copper binding and microcompartmentalization of metal.
组织化学方法与铜含量的分析测定结果并不总是具有良好的相关性，因此，金属硫蛋白（MT）的免疫反应性染色技术最近已被用于铜相关疾病的鉴别诊断。本研究比较了组织化学方法和免疫细胞化学方法在评估铜状态方面的差异。雄性大鼠喂食高铜（1 g/kg）饮食16周，并在此期间依次处死。使用原子吸收分光光度法分析肝脏和肾脏中的铜和锌，并将切片用二硫代氨基甲酸盐和罗丹宁染色以检测铜，同时使用DNP定位系统检测免疫反应性MT。MT的免疫反应性染色与铜含量的对应关系比组织化学染色更好，并且是更敏感的铜积累指标，尽管选择性较差。此外，两种方法之间细胞内染色存在明显差异，这归因于铜结合和金属微区室化的差异。
