Leijon M, Gräslund A, Nielsen P E, Buchardt O, Nordén B, Kristensen S M, Eriksson M
Department of Biophysics, Arrhenius Laboratory, Stockholm University, Sweden.
Biochemistry. 1994 Aug 23;33(33):9820-5. doi: 10.1021/bi00199a002.
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
核酸类似物肽核酸（PNA）能与互补序列的DNA杂交。通过¹H NMR研究了两种PNA-DNA双链体H-(GCTATGTC)-NH₂.d(GACATAGC)和H-(GTAGATCACT)-NH₂.d(AGTGATCTAC)的溶液结构。结果发现，PNA-DNA杂交体通过氢键形成碱基对，很可能是沃森-克里克型。从二维NOESY和COSY结果可以得出结论，PNA-DNA复合物中的DNA链采用类似B型的结构，脱氧核糖糖处于C2'-内型构象。
