Troyer D L, Goad D W, Xie H, Rohrer G A, Alexander L J, Beattie C W
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506-5602.
Cytogenet Cell Genet. 1994;67(3):199-204. doi: 10.1159/000133822.
Direct in situ single-copy polymerase chain reaction (DISC-PCR) was used to assign and orient a linkage group to pig chromosome 1. Five microsatellites were analyzed, and all five were successfully localized using this procedure. Physical data were used to orient the linkage group with respect to the centromere and estimate the amount of coverage of chromosome 1. There was excellent concordance between the physical and linkage maps. The linear order of the microsatellites was identical, and relative distances were similar. All markers were located on the long arm of chromosome 1. Coverage was estimated at about 32%. Thus, DISC-PCR rapidly and easily assigned and ordered microsatellite markers for which large genomic clones do not exist.
直接原位单拷贝聚合酶链反应（DISC-PCR）被用于将一个连锁群定位到猪的1号染色体并确定其方向。分析了五个微卫星，通过该程序所有五个微卫星都成功定位。利用物理数据确定连锁群相对于着丝粒的方向，并估计1号染色体的覆盖量。物理图谱和连锁图谱之间具有很好的一致性。微卫星的线性顺序相同，相对距离也相似。所有标记都位于1号染色体的长臂上。估计覆盖量约为32%。因此，DISC-PCR能快速且容易地对不存在大型基因组克隆的微卫星标记进行定位和排序。
