Tarallo P, Humbert J C, Mahassen P, Fournier B, Henny J
Centre de Médecine Préventive, Vandoeuvre-lès-Nancy, France.
Eur J Haematol. 1994 Jul;53(1):11-5. doi: 10.1111/j.1600-0609.1994.tb00172.x.
The reticulocyte count is a good index of erythropoietic activity. Automated methods, mainly flow cytometry, have made the counting easier, more accurate and reproducible. The data presented describe the counting of reticulocytes in 1219 apparently healthy subjects of both sexes who came for a periodic health examination. Their ages varied from 4 to over 60 years. The reticulocytes were counted with a flow cytometer (Sysmex R-3000) with Auramine-O as fluorochrome. There was no statistical difference between boys and girls aged 4-19 years. However, the reticulocyte count was significantly higher in men than in women over 20 years of age. The reticulocyte count was affected neither by the menstrual cycle in girls at puberty, nor by oral contraceptives and the menopause in women. No effect of moderate smoking was observed in either sex. Reference limits of the reticulocyte counts (percentage and absolute value) according to age and sex and the three types of reticulocytes are provided.
网织红细胞计数是红细胞生成活性的一个良好指标。自动化方法,主要是流式细胞术,使计数变得更容易、更准确且可重复。所呈现的数据描述了1219名前来进行定期健康检查的明显健康的男女受试者中网织红细胞的计数情况。他们的年龄从4岁到60多岁不等。使用以金胺 - O作为荧光染料的流式细胞仪(Sysmex R - 3000)对网织红细胞进行计数。4 - 19岁的男孩和女孩之间没有统计学差异。然而,20岁以上男性的网织红细胞计数显著高于女性。青春期女孩的月经周期、女性的口服避孕药和绝经均未影响网织红细胞计数。在任何性别中均未观察到适度吸烟的影响。提供了根据年龄、性别以及三种网织红细胞类型划分的网织红细胞计数(百分比和绝对值)的参考范围。