Vaughan W P, Hall J, Johnson K, Dougherty C, Peebles D
Am J Hematol. 1985 Apr;18(4):385-91. doi: 10.1002/ajh.2830180408.
Laser flow cytometric analysis is well suited to many clinical immunofluorescent and hematologic applications. One of these is automated reticulocyte and platelet enumerations. We have performed a clinical evaluation of a fluorescence laser flow cytometer reticulocyte and platelet enumeration method using the fluorochrome acridine orange. One hundred and thirty-two clinical specimens from inpatients in our comprehensive cancer center were analyzed for platelet counts and 101 samples from the same population were analyzed for reticulocyte counts by the flow cytometer and simultaneously by conventional techniques. Both the reticulocyte and platelet enumerations achieved a high degree of correlation with standard methods for the nonbone marrow-transplant patients studied (R = .989 for reticulocytes and R = .995 for platelets). Marrow transplant patients had a slightly poorer correlation coefficient for the reticulocyte enumeration (R = .923), but analysis of serial determinations of these patients' samples for reticulocyte counts revealed that results were more consistent over time with the automated method than with the traditional manual method. The laser flow cytometer fluorescence reticulocyte and platelet enumeration methods described are efficient, rapid, and reliable for platelet and reticulocyte counting.
激光流式细胞术分析非常适合许多临床免疫荧光和血液学应用。其中之一是自动网织红细胞和血小板计数。我们使用荧光染料吖啶橙对荧光激光流式细胞仪网织红细胞和血小板计数方法进行了临床评估。对我们综合癌症中心住院患者的132份临床标本进行了血小板计数分析，并对同一人群的101份样本进行了网织红细胞计数分析，同时采用传统技术进行分析。对于所研究的非骨髓移植患者，网织红细胞和血小板计数与标准方法均具有高度相关性（网织红细胞R = 0.989，血小板R = 0.995）。骨髓移植患者的网织红细胞计数相关系数略低（R = 0.923），但对这些患者样本进行网织红细胞计数的系列测定分析表明，与传统手工方法相比，自动方法的结果随时间更一致。所描述的激光流式细胞仪荧光网织红细胞和血小板计数方法对于血小板和网织红细胞计数是高效、快速且可靠的。
