Labia R, Philippon A, Le Goffic F, Faye J C
Biochimie. 1975;57(2):139-43. doi: 10.1016/s0300-9084(75)80163-5.
This paper is dealing with the enzymatic problem raised by two strains of Ps. aeruginosa resistant to classical beta lactam antibiotics including carbenicillin. These two strains hydrolyse all these antibiotics. In both cases, we have shown the simultaneous biosynthesis of two enzymes: an inducible and chromosome cephalosporinase frequently found in this germ, and a constitutive beta lactamase, with a penicillinase activity which has been identified with the extrachromosomic beta lactamase R-TEM. These two enzymes have been separated by affinity chromatography, characterized by their kinetic constants given by computerized microacidimetry, and their isoelectric points which are respectively 9.2 for the cephalosporinase and 5.40 for the penicillinase R-TEM. Isoelectric focussing also shows the separation of these two enzymes.
本文探讨了两株对包括羧苄青霉素在内的经典β-内酰胺抗生素耐药的铜绿假单胞菌所引发的酶学问题。这两株菌可水解所有这些抗生素。在这两种情况下，我们都证明了两种酶的同时生物合成：一种是该菌中常见的可诱导的染色体头孢菌素酶，另一种是组成型β-内酰胺酶，其具有青霉素酶活性，已被鉴定为染色体外β-内酰胺酶R-TEM。这两种酶已通过亲和层析分离，通过计算机化微量酸量法给出的动力学常数以及它们的等电点进行表征，头孢菌素酶的等电点分别为9.2，青霉素酶R-TEM的等电点为5.40。等电聚焦也显示了这两种酶的分离。
