Santer M, Santer U
J Bacteriol. 1973 Dec;116(3):1304-13. doi: 10.1128/jb.116.3.1304-1313.1973.
Two large ribonucleic acid (RNA) fragments have been obtained from T1-RNase-treated 30S ribosomes of Escherichia coli. One fragment, about 475 nucleotides long, contains all the unique oligonucleotides found by Fellner and associates in sections of 16S RNA designated P, E, E', and K, and one-half the large oligonucleotides of section A. The other large fragment is about 300 nucleotides long and contains the oligonucleotides found in sections C, C', C''. The isolation of these large fragments seems to confirm the arrangement of sections within 16S RNA. There are also recovered from nuclease-treated ribosomes three small fragments, one (120 nucleotides long) from the 5' end, one (26 nucleotides long) from the 3' OH end of the chain, and another section (66 nucleotides long) from the middle of the 16S RNA chain. Small molecular weight material is also generated by nuclease treatment, and about half this material is derived from a region close to the 3' OH end of the 16S RNA chain. This indicates that the most accessible part of the rRNA of E. coli 30S ribosomes is a region 100 to 150 nucleotides long near the 3' end of the chain. A general scheme is proposed to explain the generation of the various-sized RNA products from the rRNA of the 30S ribosome.
从经T1核糖核酸酶处理的大肠杆菌30S核糖体中获得了两个大的核糖核酸（RNA）片段。一个片段长约475个核苷酸，包含费尔纳及其同事在16S RNA的P、E、E'和K区段中发现的所有独特寡核苷酸，以及A区段大寡核苷酸的一半。另一个大片段长约300个核苷酸，包含在C、C'、C''区段中发现的寡核苷酸。这些大片段的分离似乎证实了16S RNA内各片段的排列方式。从经核酸酶处理的核糖体中还回收了三个小片段，一个（120个核苷酸长）来自5'端，一个（26个核苷酸长）来自链的3' OH端，另一个区段（66个核苷酸长）来自16S RNA链的中部。核酸酶处理还产生了小分子质量物质，其中约一半物质来自靠近16S RNA链3' OH端的区域。这表明大肠杆菌30S核糖体rRNA最易接近的部分是靠近链3'端的一个100至150个核苷酸长的区域。提出了一个总体方案来解释从30S核糖体的rRNA产生各种大小RNA产物的过程。
