Gallay S H, Miura Y, Commisso C N, Fitzsimmons J S, O'Driscoll S W
Cartilage and Connective Tissue Research Laboratory, Mayo Clinic, Rochester, Minnesota 55905.
J Orthop Res. 1994 Jul;12(4):515-25. doi: 10.1002/jor.1100120408.
Periosteum has been shown in vitro and in vivo to have a chondrogenic potential that permits it to be used for cartilage regeneration. A useful donor site should have good chondrogenic potential, availability of a large quantity of periosteum, and relative ease of access, and it should be associated with a low rate of morbidity. We hypothesized that the chondrogenic potential of periosteum varies from one bone to another and among different regions of the periosteum from a single bone. A total of 370 periosteal and 37 fascia lata (control) explants were taken from the skull, the ilium, the scapula, the upper, middle, and lower medial proximal tibia, the posterior proximal tibia, and the distal tibia of 2-month-old New Zealand rabbits. The explants were cultured for 6 weeks in agarose/Dulbecco's modified Eagle medium to which 10 ng/ml of transforming growth factor-beta 1 was added during the first 2 weeks. Skeletal muscle and fascia lata were used as controls. In addition, the thickness, cell density, and total cell count of the cambium layer were measured in 24 explants from the donor sites on the ilium and the upper, middle, and lower proximal tibia. At 6 weeks, histomorphometry and quantitative collagen typing were performed. The periosteal donor sites could be grouped into three categories according to chondrogenic potential: ilium (best), scapula and tibia, and skull (no chondrogenesis). The scapular periosteum was slightly better than that from the tibia. Within the tibia, the upper and middle zones of the proximal region were similar and were slightly better than the lower proximal tibia or the distal tibia. The cellularity of the cambium layer correlated positively with the amount of cartilage as a percentage of the total area. The results of this study indicate that iliac periosteum exhibited the best overall chondrogenic potential in vitro but that periosteum from the traditionally used medial proximal tibia also was excellent. Periosteum from the skull was not chondrogenic. The chondrogenic potential of periosteum varies from bone to bone and within the periosteum from one bone. This variation in chondrogenic potential among donor sites may be due to a difference in the total cell count of the cambium layer.
骨膜已在体外和体内被证明具有软骨生成潜能，这使得它可用于软骨再生。一个有用的供区应具有良好的软骨生成潜能、大量骨膜的可获取性、相对容易的取材途径，并且应与低发病率相关。我们假设骨膜的软骨生成潜能在不同骨之间以及同一骨的骨膜不同区域之间存在差异。从2月龄新西兰兔的颅骨、髂骨、肩胛骨、胫骨近端内侧上、中、下区域、胫骨近端后侧以及胫骨远端共获取了370个骨膜外植体和37个阔筋膜（对照）外植体。将外植体在琼脂糖/杜氏改良 Eagle 培养基中培养6周，在前2周向培养基中添加10 ng/ml 的转化生长因子-β1。骨骼肌和阔筋膜用作对照。此外，在取自髂骨以及胫骨近端上、中、下区域供区的24个外植体中测量了生发层的厚度、细胞密度和总细胞数。在6周时，进行了组织形态计量学和定量胶原分型。根据软骨生成潜能，骨膜供区可分为三类：髂骨（最佳）、肩胛骨和胫骨，以及颅骨（无软骨生成）。肩胛骨膜略优于胫骨骨膜。在胫骨内，近端区域的上、中区域相似，且略优于胫骨近端下部或胫骨远端。生发层的细胞密度与软骨占总面积的百分比呈正相关。本研究结果表明，髂骨骨膜在体外表现出最佳的总体软骨生成潜能，但传统使用的胫骨近端内侧骨膜也非常出色。颅骨骨膜不具有软骨生成能力。骨膜的软骨生成潜能在不同骨之间以及同一骨的骨膜内存在差异。供区之间这种软骨生成潜能的差异可能归因于生发层总细胞数的不同。
