斑疹伤寒立克次体对绵羊红细胞血影的吸附作用。
Adsorption of typhus rickettsiae to ghosts of sheep erythrocytes.
作者信息
Winkler H H, Ramm L E
出版信息
Infect Immun. 1975 Jun;11(6):1244-51. doi: 10.1128/iai.11.6.1244-1251.1975.
Abstract
Rickettsia prowazeki will adsorb to ghosts derived from sheep erythrocytes by hypotonic lysis. Adsorption to ghosts, as to intact erythrocytes, is dependent on the metabolism of the rickettsiae. KCN and 0 C inhibit adsorption. Fluoride, while inhibiting hemolysis, has no effect on adsorption to ghosts or intact erythrocytes. The adsorption of rickettsiae to ghosts does not lead to lysis, in that fluorescent albumin can be retained by the ghost after adsorption. Both inside-out and right-side-out vesicles formed from the ghost are equally capable of adsorption, indicating that the receptor is stable during the process of vesiculation and is not localized to one aspect of the cell membrane. Adsorption to ghosts is slower and displays less affinity than to the intact erythrocyte. The ghost system will be useful in characterizing the membrane receptor for rickettsia and in establishing assays for adsorption to other cells.
摘要
普氏立克次氏体可通过低渗裂解吸附于源自绵羊红细胞的血影。与吸附完整红细胞一样，吸附于血影也依赖于立克次氏体的代谢。氰化钾和0℃抑制吸附。氟化物虽抑制溶血，但对吸附于血影或完整红细胞无影响。立克次氏体吸附于血影不会导致裂解，因为吸附后荧光白蛋白可被血影保留。由血影形成的内翻和外翻囊泡均同样具有吸附能力，这表明受体在囊泡化过程中是稳定的，且并非定位于细胞膜的某一侧。与完整红细胞相比，吸附于血影的速度较慢且亲和力较低。血影系统将有助于表征立克次氏体的膜受体，并建立针对其他细胞吸附的检测方法。
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本文引用的文献
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