COHN Z A, BOZEMAN F M, CAMPBELL J M, HUMPHRIES J W, SAWYER T K
J Exp Med. 1959 Mar 1;109(3):271-92. doi: 10.1084/jem.109.3.271.
A system has been described in which the penetration of Rickettsia tsutsugamushi (Karp strain) into tissue culture cells can be quantitated, and the factors affecting this process studied. The results indicated that rickettsial penetration in vitro depended largely on the viability of the organisms. Certain components of the fluid environment such as the divalent cations and protein were found to be of importance. The temperature dependence of the penetration process was found to vary with the nature of the suspending medium. A number of compounds related to L-glutamic acid enhanced penetration, whereas metabolic inhibitors depressed this process. Aureomycin at concentrations between 50 and 250 microg./ml. inhibited the penetration of rickettsiae while chloramphenicol at similar concentrations was ineffective. The results are discussed in terms of the biological and biochemical properties of this group of agents.
已描述了一种系统，在该系统中可对恙虫病立克次体（Karp株）侵入组织培养细胞的情况进行定量，并研究影响这一过程的因素。结果表明，立克次体在体外的侵入很大程度上取决于微生物的活力。发现液体环境的某些成分，如二价阳离子和蛋白质很重要。发现侵入过程对温度的依赖性随悬浮介质的性质而变化。一些与L-谷氨酸有关的化合物可增强侵入，而代谢抑制剂则会抑制这一过程。浓度在50至250微克/毫升之间的金霉素可抑制立克次体的侵入，而浓度相似的氯霉素则无效。根据这类病原体的生物学和生化特性对结果进行了讨论。
