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钝顶螺旋藻耐氯酸盐突变体的分离与鉴定

Isolation and characterization of a chlorate-resistant mutant of Spirulina platensis.

作者信息

Lanfaloni L, Cappanna E, Gualerzi C O

机构信息

Institute of Cell Biology, University of Perugia, Italy.

出版信息

New Microbiol. 1994 Apr;17(2):133-40.

PMID:8065270
Abstract

Three chlorate-resistant mutants of the cyanobacterium Spirulina platensis were obtained by UV irradiation and one of them (LL1) was further characterized for its nutritional requirements and for the capacity to reduce nitrate in vivo and in vitro. The results indicate that mutation leading to chlorate resistance is not due to inactivation of nitrate reductase but is most likely due to the loss of permeability to chlorate and nitrate. The other two mutants seem to have properties similar to those of LL1.

摘要

通过紫外线照射获得了钝顶螺旋藻的三个抗氯酸盐突变体,其中一个(LL1)进一步针对其营养需求以及在体内和体外还原硝酸盐的能力进行了表征。结果表明,导致抗氯酸盐的突变并非由于硝酸还原酶失活,而极有可能是由于对氯酸盐和硝酸盐的通透性丧失。另外两个突变体似乎具有与LL1相似的特性。

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