Hughes J E, Kiyosawa H, Welker D L
Department of Biology, Utah State University, Logan 84322-5500.
Mol Cell Biol. 1994 Sep;14(9):6117-24. doi: 10.1128/mcb.14.9.6117-6124.1994.
All of the plasmid-carried genes expressed during vegetative growth are essential for long-term maintenance of plasmid Ddp1 in the nucleus of Dictyostelium discoideum. Deletion of Ddp1 genes expressed only during development had no detectable effect on plasmid maintenance. Deletion of vegetatively expressed genes, either singly or in pairs, resulted in (i) a rapid loss of plasmid from cells grown in the absence of selection for plasmid retention, (ii) variation in the proportion of monomer to multimer forms of the plasmid molecules, and/or (iii) abnormalities in plasmid copy number. At least two plasmid-encoded gene products influence patterns of expression of plasmid genes.
在营养生长期间表达的所有质粒携带基因对于盘基网柄菌细胞核中质粒Ddp1的长期维持至关重要。仅在发育期间表达的Ddp1基因的缺失对质粒维持没有可检测到的影响。单独或成对缺失营养生长期间表达的基因会导致：（i）在没有选择保留质粒的情况下生长的细胞中质粒迅速丢失；（ii）质粒分子单体与多聚体形式的比例发生变化；和/或（iii）质粒拷贝数异常。至少两种质粒编码的基因产物影响质粒基因的表达模式。
