Gurniak C B, Bang A G, Noegel A A
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Curr Genet. 1990 Apr;17(4):321-5. doi: 10.1007/BF00314879.
The sequence of a 5.1 kb contiguous fragment of the Dictyostelium plasmid Ddp1 is presented. This fragment contains three long open reading frames which correspond to the developmentally regulated and cAMP-inducible transcript d-5, the growth phase specific transcript g-1 and the three overlapping transcripts g-2, g-3 and d-4. The transcripts that originate from Ddp1 resemble chromosomally-encoded ones: they are products of RNA polymerase II, are polyadenylated and accumulate at different time points during Dictyostelium development. The presented nucleotide sequence encompasses a 2,033 bp HindIII fragment that had previously been shown to carry all the information necessary for extrachromosomal replication. None of the identified genes is completely contained within this HindIII fragment.
本文给出了盘基网柄菌质粒Ddp1的一个5.1 kb连续片段的序列。该片段包含三个长开放阅读框，分别对应发育调控且cAMP诱导的转录本d - 5、生长阶段特异性转录本g - 1以及三个重叠转录本g - 2、g - 3和d - 4。源自Ddp1的转录本类似于染色体编码的转录本：它们是RNA聚合酶II的产物，进行多聚腺苷酸化，并在盘基网柄菌发育的不同时间点积累。所呈现的核苷酸序列包含一个2033 bp的HindIII片段，该片段先前已被证明携带了染色体外复制所需的所有信息。所鉴定的基因均未完全包含在这个HindIII片段内。
