[来自类新凶手弗朗西斯菌F6168菌株的隐蔽质粒的分离及分子遗传学特征]
[Isolation and molecular-genetic characteristic of a cryptic plasmid from the Francisella novicida like F6168 strain].
作者信息
Pavlov V M, Rodionova I V, Mokrievich A N, Meshcheriakova I S
出版信息
Mol Gen Mikrobiol Virusol. 1994 May-Jun(3):39-40.
PMID:8065386
Abstract
A 4 Kb plasmid DNA has been isolated from Francisella novicida like strain F6168. Restriction map of the plasmid was constructed for restriction endonucleases HindIII, XbaI, EcoRV, BgIII. The plasmid pFN10 has been shown to be stably inherited by F. tularensis. The use of pFN10 for the construction of plasmid vectors for microorganisms of the genus Francisella is discussed.
摘要
已从类新凶手弗朗西斯菌菌株F6168中分离出一段4千碱基的质粒DNA。构建了该质粒针对限制性内切酶HindIII、XbaI、EcoRV、BgIII的限制性图谱。已证明质粒pFN10可被土拉弗朗西斯菌稳定遗传。文中讨论了使用pFN10构建弗朗西斯菌属微生物质粒载体的情况。
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