Ludu Jagjit S, Nix Eli B, Duplantis Barry N, de Bruin Olle M, Gallagher Larry A, Hawley Laura M, Nano Francis E
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
FEMS Microbiol Lett. 2008 Jan;278(1):86-93. doi: 10.1111/j.1574-6968.2007.00979.x. Epub 2007 Nov 15.
Francisella novicida is a gram-negative pathogen that can induce disease in mice that mimics human tularemia, and is nearly identical to Francisella tularensis at the genomic level. In this work a number of antibiotic marker cassettes that incorporate a strong F. novicida promoter is constructed, which greatly enhances selection in F. novicida and F. tularensis. Two low-copy plasmid vectors based on a broad-host-range plasmid, and an integrating vector have also been made, and these can be used for genetic complementation. Two general approaches to deletion mutagenesis in F. novicida is also described.
新凶手弗朗西斯菌是一种革兰氏阴性病原体，可在小鼠中诱发类似于人类兔热病的疾病，并且在基因组水平上与土拉弗朗西斯菌几乎相同。在这项工作中，构建了许多包含强新凶手弗朗西斯菌启动子的抗生素标记盒，这大大增强了在新凶手弗朗西斯菌和土拉弗朗西斯菌中的筛选。还制备了基于广宿主范围质粒的两种低拷贝质粒载体和一种整合载体，这些可用于基因互补。本文还描述了新凶手弗朗西斯菌中缺失诱变的两种一般方法。
