Hardy P M, Holland D, Scott S, Garman A J, Newton C R, McLean M J
Cambridge Research Biochemicals, Northwich, Cheshire, UK.
Nucleic Acids Res. 1994 Aug 11;22(15):2998-3004. doi: 10.1093/nar/22.15.2998.
In order to increase the efficiency of use of automated DNA synthesizers (i.e. the number of oligomers prepared per day), we have devised and prepared novel phosphoramidite reagents that contain a linking group which, while stable under the normal synthesis conditions, is cleaved under basic conditions. When one of these linkers is introduced at the desired position in the synthesis of an oligonucleotide, subsequent detritylation enables the synthesis of a second oligonucleotides sequence upon the first. During deprotection of the oligonucleotide with ammonium hydroxide, the chain is cleaved at either side of the points of introduction of the novel reagent, generating two oligonucleotides free in solution. These reagents are of particular use in applications where oligomers are used in pairs (such as PCR, chemical synthesis of genes etc.) and means that an automated synthesis facility can be used more efficiently, without the need for operator intervention, after the working day is over.
为了提高自动化DNA合成仪的使用效率（即每天制备的寡聚物数量），我们设计并制备了新型亚磷酰胺试剂，该试剂含有一个连接基团，该连接基团在正常合成条件下稳定，但在碱性条件下会裂解。当在寡核苷酸合成的所需位置引入这些连接子之一时，随后的脱三苯甲基化能够在第一个寡核苷酸序列上合成第二个寡核苷酸序列。在用氢氧化铵对寡核苷酸进行脱保护过程中，链在新型试剂引入点的两侧裂解，产生两个在溶液中游离的寡核苷酸。这些试剂在寡聚物成对使用的应用（如PCR、基因化学合成等）中特别有用，这意味着在工作日结束后，无需操作人员干预，自动化合成设备就能更高效地使用。
