Gait M J
Medical Research Council, Cambridge, UK.
Curr Opin Biotechnol. 1991 Feb;2(1):61-8. doi: 10.1016/0958-1669(91)90062-a.
Reliable methods of machine-aided RNA synthesis have been established to complement those for DNA assembly. Oligonucleotides containing thio-modified backbones and 2'-O-alkyl sugars head the list of many newly available analogues. Biotin, fluorescent agents and many reporter groups can be conveniently introduced into oligonucleotides in multiples by phosphoramidite or H-phosphonate chemistry.
已经建立了可靠的机器辅助RNA合成方法，以补充DNA组装方法。含有硫代修饰主链和2'-O-烷基糖的寡核苷酸位居许多新可用类似物之首。生物素、荧光剂和许多报告基团可以通过亚磷酰胺或H-膦酸酯化学方便地以多种方式引入寡核苷酸中。
