钠离子浓度对单链DNA链内碱基配对的影响。
The effect of sodium ion concentration on intrastrand base-pairing in single-stranded DNA.
作者信息
Wolfe A R, Meehan T
机构信息
Division of Toxicology, University of California, San Francisco 94143.
出版信息
Nucleic Acids Res. 1994 Aug 11;22(15):3147-50. doi: 10.1093/nar/22.15.3147.
Abstract
The salt-induced formation of duplex structure (primarily hairpin loops) in denatured calf thymus DNA was monitored by measuring the decrease in absorbance at 260 nm as a function of increasing sodium ion concentration. It was found that this process was noncooperative and could be accurately described by the mass-action expression for the reversible formation of a binary complex: single strand (coil) + free sodium ion <==> hairpin (with associated sodium ion). The equilibrium constant for the transition was found to be 6 (M Na+)-1. The extrapolated absorbance at infinite salt concentration represents 11% hyperchromicity, which is one third of the hyperchromicity of denatured DNA in the absence of salt (36%).
摘要
通过测量260nm处吸光度随钠离子浓度增加的降低情况，监测了盐诱导变性小牛胸腺DNA中双链结构（主要是发夹环）的形成。发现该过程是非协同的，并且可以用二元复合物可逆形成的质量作用表达式准确描述：单链（线圈）+游离钠离子⇌发夹（与相关钠离子）。发现转变的平衡常数为6（M Na⁺）⁻¹。无限盐浓度下的外推吸光度代表11%的增色性，这是无盐时变性DNA增色性（36%）的三分之一。
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