DiGiacomo R F, Deeb B J, Brodie S J, Zimmerman T E, Veltkamp E R, Chrisp C E
Department of Comparative Medicine, School of Medicine, University of Washington, Seattle 98195.
Am J Vet Res. 1993 Aug;54(8):1280-6.
Naturally acquired turbinate atrophy in rabbits was associated with Pasteurella multocida infection. Several in vitro and in vivo studies were conducted to document toxin production from P multocida isolates and to determine the relation of toxin to atrophic rhinitis in rabbits. Ten isolates of P multocida serotype A:12 were obtained from adult New Zealand White rabbits with noninduced atrophic rhinitis. Specific-pathogen-free rabbits inoculated intranasally with isolates of P multocida developed rhinitis and turbinate atrophy. However, inoculation with filtrates of the same bacteria failed to induce turbinate atrophy. Cytotoxicity was observed in assays, using bovine embryonic turbinate cell cultures with extracts of P multocida, but not in agar overlay cytotoxicity assays, using bovine embryonic turbinate, bovine embryonic lung, or Vero cell cultures, or in a sandwich ELISA, using monoclonal antibodies to purified P multocida toxin. Thus, turbinate atrophy was experimentally reproduced in rabbits with isolates of P multocida, but toxin was only detected in vitro by cell culture assay of P multocida extracts.
家兔自然发生的鼻甲萎缩与多杀巴斯德菌感染有关。进行了多项体外和体内研究，以记录多杀巴斯德菌分离株产生毒素的情况，并确定毒素与家兔萎缩性鼻炎的关系。从患有非诱发性萎缩性鼻炎的成年新西兰白兔中获得了10株多杀巴斯德菌A:12血清型分离株。经鼻接种多杀巴斯德菌分离株的无特定病原体家兔出现了鼻炎和鼻甲萎缩。然而，接种相同细菌的滤液未能诱发鼻甲萎缩。在使用牛胚胎鼻甲细胞培养物和多杀巴斯德菌提取物的试验中观察到细胞毒性，但在使用牛胚胎鼻甲、牛胚胎肺或Vero细胞培养物的琼脂覆盖细胞毒性试验中，以及在使用针对纯化的多杀巴斯德菌毒素的单克隆抗体的夹心ELISA试验中均未观察到细胞毒性。因此，用多杀巴斯德菌分离株在家兔中实验性再现了鼻甲萎缩，但仅通过对多杀巴斯德菌提取物进行细胞培养测定在体外检测到了毒素。
