Fehling H J, Swat W, Laplace C, Kühn R, Rajewsky K, Müller U, von Boehmer H
Basel Institute for Immunology, Switzerland.
Science. 1994 Aug 26;265(5176):1234-7. doi: 10.1126/science.8066463.
Proteasomes degrade endogenous proteins. Two subunits, LMP-2 and LMP-7, are encoded in a region of the major histocompatibility complex (MHC) that is critical for class I-restricted antigen presentation. Mice with a targeted deletion of the gene encoding LMP-7 have reduced levels of MHC class I cell-surface expression and present the endogenous antigen HY inefficiently; addition of peptides to splenocytes deficient in LMP-7 restores wild-type class I expression levels. This demonstrates the involvement of LMP-7 in the MHC class I presentation pathway and suggests that LMP-7 functions as an integral part of the peptide supply machinery.
蛋白酶体可降解内源性蛋白质。其中两个亚基，即LMP - 2和LMP - 7，由主要组织相容性复合体（MHC）的一个区域编码，该区域对于I类限制性抗原呈递至关重要。靶向缺失编码LMP - 7基因的小鼠，其MHC I类细胞表面表达水平降低，且内源性抗原HY呈递效率低下；向缺乏LMP - 7的脾细胞中添加肽可恢复野生型I类表达水平。这证明了LMP - 7参与MHC I类呈递途径，并表明LMP - 7作为肽供应机制的一个组成部分发挥作用。
