Martinez D, Maillard J C, Coisne S, Sheikboudou C, Bensaid A
Departement Elevage et Médecine Vétérinaire des Pays Tropicaux, Centre International en Recherche Agronomique pour le Développement (EMVT-CIRAD), Pointe à Pitre, Guadeloupe.
Vet Immunol Immunopathol. 1994 May;41(1-2):153-63. doi: 10.1016/0165-2427(94)90064-7.
In two experiments, four and five goats were vaccinated by giving two subcutaneous injections of a preparation of inactivated elementary bodies of Cowdria ruminantium (Gardel stock) mixed with Freund's adjuvant. All vaccinated animals together with four naive controls were challenged intravenously with 5 ml of supernatant of a culture of bovine endothelial cells infected with the same stock of Cowdria. All goats developed a high temperature. Two out of four, and four out of five vaccinated goats survived the challenge whereas all naive control animals died within 7-12 days. Vaccinated goats which died survived longer than the controls. No difference in antibody titres was observed between protected and non-protected vaccinated goats. Moreover, immune sera from surviving goats, whether heat inactivated or not, were unable to neutralize the infection of bovine endothelial cells by Cowdria in vitro. Mechanisms conferring protection on the immunized goats are unknown at the moment but the hypothesis that T-helper lymphocyte populations have been elicited seems to be likely. This method of immunization with dead organisms will help in the search for protective antigens against cowdriosis.
在两项实验中，分别给4只和5只山羊进行皮下注射，注射的是与弗氏佐剂混合的反刍兽考德里氏体（加德尔株）灭活原体制剂。所有接种疫苗的动物以及4只未接种疫苗的对照动物，通过静脉注射5毫升感染了相同考德里氏体株的牛内皮细胞培养上清液进行攻毒。所有山羊均出现高热。4只接种疫苗的山羊中有2只、5只接种疫苗的山羊中有4只在攻毒后存活下来，而所有未接种疫苗的对照动物在7至12天内死亡。死亡的接种疫苗山羊比对照动物存活时间更长。在受到保护和未受保护的接种疫苗山羊之间未观察到抗体滴度的差异。此外，存活山羊的免疫血清，无论是否经过热灭活，在体外均无法中和考德里氏体对牛内皮细胞的感染。目前尚不清楚赋予免疫山羊保护作用的机制，但T辅助淋巴细胞群体被激活的假说似乎很有可能成立。这种用死菌进行免疫的方法将有助于寻找抗牛巴贝斯虫病的保护性抗原。
